Identification of the sulfolipids in the non-photosynthetic diatom nitzschia alba

R. Anderson; M. Kates; B. E. Volcani

doi:10.1016/0005-2760(78)90055-3

Identification of the sulfolipids in the non-photosynthetic diatom nitzschia alba

R. Anderson, M. Kates, B. E. Volcani

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Abstract

The four major sulfolipids in the non-photosynthetic marine diatom, Nitzschia alba, were isolated in pure form and their structures were established spectrometrically and by identification of their hydrolysis products as (a) 24-methylene cholesterol sulfate, (b) 1-deoxyceramide-1-sulfonate, (c) phosphatidyl sulfocholine (a sulfonium analogue of phosphatidylcholine) and (d) sulfoquinovosyi diglyceride. The major characteristic fatty acids of the sulfolipids were: for the deoxyceramide sulfonate, 16:0 (26%) and 16:1-Δ³-trans (64%); for the sulfonium analogue, 14:0 (30%), 18:1 (12%), 18:2 (8%), 20:5 (27%) and 22:6 (4%); and for the sulfoquinovosyl diglyceride (two species, respectively), 14:0 (9%, 22%), 16:0 (16%, 28%), 18:1 (8%, 22%), 20:5 (42%, 23%) and 22:6 (14%, 2%). Traces of lyso-derivatives of sulfoquinovosyl diglyceride and phosphatidyl sulfocholine were also detected. The deoxyceramide sulfonate and the phosphatidyl sulfocholine represent novel membrane lipid components not previously detected in other organisms. They may however have a widespread distribution in marine diatoms and perhaps in marine organisms generally.

Original language	English
Pages (from-to)	89-106
Number of pages	18
Journal	Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume	528
Issue number	1
DOIs	https://doi.org/10.1016/0005-2760(78)90055-3
Publication status	Published - Jan 27 1978
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by grants from NRC of Canada (A-5324) and from NIH (GM-08229-13-14). The authors are indebted to Drs. Gordon W. Wood and Pui-Yan Lau for the field desorption mass spectra, to Dr. Ian P. Smith for the 13CN MR spectra and to Mr. Hector Seguin for the microanalyses.

ASJC Scopus Subject Areas

Biophysics
Biochemistry
Endocrinology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0005-2760(78)90055-3

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abstract = "The four major sulfolipids in the non-photosynthetic marine diatom, Nitzschia alba, were isolated in pure form and their structures were established spectrometrically and by identification of their hydrolysis products as (a) 24-methylene cholesterol sulfate, (b) 1-deoxyceramide-1-sulfonate, (c) phosphatidyl sulfocholine (a sulfonium analogue of phosphatidylcholine) and (d) sulfoquinovosyi diglyceride. The major characteristic fatty acids of the sulfolipids were: for the deoxyceramide sulfonate, 16:0 (26%) and 16:1-Δ3-trans (64%); for the sulfonium analogue, 14:0 (30%), 18:1 (12%), 18:2 (8%), 20:5 (27%) and 22:6 (4%); and for the sulfoquinovosyl diglyceride (two species, respectively), 14:0 (9%, 22%), 16:0 (16%, 28%), 18:1 (8%, 22%), 20:5 (42%, 23%) and 22:6 (14%, 2%). Traces of lyso-derivatives of sulfoquinovosyl diglyceride and phosphatidyl sulfocholine were also detected. The deoxyceramide sulfonate and the phosphatidyl sulfocholine represent novel membrane lipid components not previously detected in other organisms. They may however have a widespread distribution in marine diatoms and perhaps in marine organisms generally.",

author = "R. Anderson and M. Kates and Volcani, {B. E.}",

note = "Funding Information: This work was supported by grants from NRC of Canada (A-5324) and from NIH (GM-08229-13-14). The authors are indebted to Drs. Gordon W. Wood and Pui-Yan Lau for the field desorption mass spectra, to Dr. Ian P. Smith for the 13CN MR spectra and to Mr. Hector Seguin for the microanalyses.",

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doi = "10.1016/0005-2760(78)90055-3",

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AU - Kates, M.

AU - Volcani, B. E.

N1 - Funding Information: This work was supported by grants from NRC of Canada (A-5324) and from NIH (GM-08229-13-14). The authors are indebted to Drs. Gordon W. Wood and Pui-Yan Lau for the field desorption mass spectra, to Dr. Ian P. Smith for the 13CN MR spectra and to Mr. Hector Seguin for the microanalyses.

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N2 - The four major sulfolipids in the non-photosynthetic marine diatom, Nitzschia alba, were isolated in pure form and their structures were established spectrometrically and by identification of their hydrolysis products as (a) 24-methylene cholesterol sulfate, (b) 1-deoxyceramide-1-sulfonate, (c) phosphatidyl sulfocholine (a sulfonium analogue of phosphatidylcholine) and (d) sulfoquinovosyi diglyceride. The major characteristic fatty acids of the sulfolipids were: for the deoxyceramide sulfonate, 16:0 (26%) and 16:1-Δ3-trans (64%); for the sulfonium analogue, 14:0 (30%), 18:1 (12%), 18:2 (8%), 20:5 (27%) and 22:6 (4%); and for the sulfoquinovosyl diglyceride (two species, respectively), 14:0 (9%, 22%), 16:0 (16%, 28%), 18:1 (8%, 22%), 20:5 (42%, 23%) and 22:6 (14%, 2%). Traces of lyso-derivatives of sulfoquinovosyl diglyceride and phosphatidyl sulfocholine were also detected. The deoxyceramide sulfonate and the phosphatidyl sulfocholine represent novel membrane lipid components not previously detected in other organisms. They may however have a widespread distribution in marine diatoms and perhaps in marine organisms generally.

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Identification of the sulfolipids in the non-photosynthetic diatom nitzschia alba

Abstract

Bibliographical note

ASJC Scopus Subject Areas

UN SDGs

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