Identification of the sulfolipids in the non-photosynthetic diatom nitzschia alba

R. Anderson; M. Kates; B. E. Volcani

doi:10.1016/0005-2760(78)90055-3

Identification of the sulfolipids in the non-photosynthetic diatom nitzschia alba

R. Anderson, M. Kates, B. E. Volcani

Résultat de recherche: Article › examen par les pairs

71 Citations (Scopus)

Résumé

The four major sulfolipids in the non-photosynthetic marine diatom, Nitzschia alba, were isolated in pure form and their structures were established spectrometrically and by identification of their hydrolysis products as (a) 24-methylene cholesterol sulfate, (b) 1-deoxyceramide-1-sulfonate, (c) phosphatidyl sulfocholine (a sulfonium analogue of phosphatidylcholine) and (d) sulfoquinovosyi diglyceride. The major characteristic fatty acids of the sulfolipids were: for the deoxyceramide sulfonate, 16:0 (26%) and 16:1-Δ³-trans (64%); for the sulfonium analogue, 14:0 (30%), 18:1 (12%), 18:2 (8%), 20:5 (27%) and 22:6 (4%); and for the sulfoquinovosyl diglyceride (two species, respectively), 14:0 (9%, 22%), 16:0 (16%, 28%), 18:1 (8%, 22%), 20:5 (42%, 23%) and 22:6 (14%, 2%). Traces of lyso-derivatives of sulfoquinovosyl diglyceride and phosphatidyl sulfocholine were also detected. The deoxyceramide sulfonate and the phosphatidyl sulfocholine represent novel membrane lipid components not previously detected in other organisms. They may however have a widespread distribution in marine diatoms and perhaps in marine organisms generally.

Langue d'origine	English
Pages (de-à)	89-106
Nombre de pages	18
Journal	Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume	528
Numéro de publication	1
DOI	https://doi.org/10.1016/0005-2760(78)90055-3
Statut de publication	Published - janv. 27 1978
Publié à l'externe	Oui

Note bibliographique

Funding Information:
This work was supported by grants from NRC of Canada (A-5324) and from NIH (GM-08229-13-14). The authors are indebted to Drs. Gordon W. Wood and Pui-Yan Lau for the field desorption mass spectra, to Dr. Ian P. Smith for the 13CN MR spectra and to Mr. Hector Seguin for the microanalyses.

ASJC Scopus Subject Areas

Biophysics
Biochemistry
Endocrinology

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10.1016/0005-2760(78)90055-3

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title = "Identification of the sulfolipids in the non-photosynthetic diatom nitzschia alba",

abstract = "The four major sulfolipids in the non-photosynthetic marine diatom, Nitzschia alba, were isolated in pure form and their structures were established spectrometrically and by identification of their hydrolysis products as (a) 24-methylene cholesterol sulfate, (b) 1-deoxyceramide-1-sulfonate, (c) phosphatidyl sulfocholine (a sulfonium analogue of phosphatidylcholine) and (d) sulfoquinovosyi diglyceride. The major characteristic fatty acids of the sulfolipids were: for the deoxyceramide sulfonate, 16:0 (26%) and 16:1-Δ3-trans (64%); for the sulfonium analogue, 14:0 (30%), 18:1 (12%), 18:2 (8%), 20:5 (27%) and 22:6 (4%); and for the sulfoquinovosyl diglyceride (two species, respectively), 14:0 (9%, 22%), 16:0 (16%, 28%), 18:1 (8%, 22%), 20:5 (42%, 23%) and 22:6 (14%, 2%). Traces of lyso-derivatives of sulfoquinovosyl diglyceride and phosphatidyl sulfocholine were also detected. The deoxyceramide sulfonate and the phosphatidyl sulfocholine represent novel membrane lipid components not previously detected in other organisms. They may however have a widespread distribution in marine diatoms and perhaps in marine organisms generally.",

author = "R. Anderson and M. Kates and Volcani, {B. E.}",

note = "Funding Information: This work was supported by grants from NRC of Canada (A-5324) and from NIH (GM-08229-13-14). The authors are indebted to Drs. Gordon W. Wood and Pui-Yan Lau for the field desorption mass spectra, to Dr. Ian P. Smith for the 13CN MR spectra and to Mr. Hector Seguin for the microanalyses.",

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doi = "10.1016/0005-2760(78)90055-3",

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AU - Kates, M.

AU - Volcani, B. E.

N1 - Funding Information: This work was supported by grants from NRC of Canada (A-5324) and from NIH (GM-08229-13-14). The authors are indebted to Drs. Gordon W. Wood and Pui-Yan Lau for the field desorption mass spectra, to Dr. Ian P. Smith for the 13CN MR spectra and to Mr. Hector Seguin for the microanalyses.

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N2 - The four major sulfolipids in the non-photosynthetic marine diatom, Nitzschia alba, were isolated in pure form and their structures were established spectrometrically and by identification of their hydrolysis products as (a) 24-methylene cholesterol sulfate, (b) 1-deoxyceramide-1-sulfonate, (c) phosphatidyl sulfocholine (a sulfonium analogue of phosphatidylcholine) and (d) sulfoquinovosyi diglyceride. The major characteristic fatty acids of the sulfolipids were: for the deoxyceramide sulfonate, 16:0 (26%) and 16:1-Δ3-trans (64%); for the sulfonium analogue, 14:0 (30%), 18:1 (12%), 18:2 (8%), 20:5 (27%) and 22:6 (4%); and for the sulfoquinovosyl diglyceride (two species, respectively), 14:0 (9%, 22%), 16:0 (16%, 28%), 18:1 (8%, 22%), 20:5 (42%, 23%) and 22:6 (14%, 2%). Traces of lyso-derivatives of sulfoquinovosyl diglyceride and phosphatidyl sulfocholine were also detected. The deoxyceramide sulfonate and the phosphatidyl sulfocholine represent novel membrane lipid components not previously detected in other organisms. They may however have a widespread distribution in marine diatoms and perhaps in marine organisms generally.

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Identification of the sulfolipids in the non-photosynthetic diatom nitzschia alba

Résumé

Note bibliographique

ASJC Scopus Subject Areas

ODD de l’ONU

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