Metabolic resistance to organophosphate insecticides in natural populations of the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) in Egypt and molecular identification of mitotypes

El Sayed H. Shaurub; Jorge R. Paredes-Montero; Judith K. Brown; Haggag S. Zein; Amr A. Mohamed

doi:10.1007/s12600-020-00858-9

Metabolic resistance to organophosphate insecticides in natural populations of the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) in Egypt and molecular identification of mitotypes

El Sayed H. Shaurub, Jorge R. Paredes-Montero, Judith K. Brown, Haggag S. Zein, Amr A. Mohamed

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

The whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) cryptic species has become an important pest of cotton and vegetable crops in Egypt. In this study, resistance to three organophosphate insecticides (OPs), chlorpyrifos-methyl, profenofos and pirimiphos-methyl, and detoxification enzymatic activities of acetylcholinesterase, carboxylesterase, and monooxygenase were evaluated to establish baseline resistance levels for B. tabaci infesting cotton in seven Egyptian governorates, compared to a susceptible laboratory reference B. tabaci colony. Resistance to OPs ranged from low-to-moderate or low-to-high, while detoxification enzymatic activities were predominantly governorate-dependent. Phylogenetic analysis of a fragment of the mitochondria cytochrome oxidase I gene (756 bp) identified field-collected B. tabaci as the B mitotype. The results provide baseline OP resistance levels for B. tabaci that will aid in interpreting future monitoring outcomes and enable detection of insecticide resistance fluxes.

Original language	English
Pages (from-to)	443-457
Number of pages	15
Journal	Phytoparasitica
Volume	49
Issue number	3
DOIs	https://doi.org/10.1007/s12600-020-00858-9
Publication status	Published - Jul 2021
Externally published	Yes

Bibliographical note

Funding Information:
The authors extend appreciation to Prof. Abdelhadi Abdallah, Department of Genetics, Faculty of Agriculture, Cairo University for providing whitefly colonies, to Dr. Mustafa M. Soliman, Department of Entomology, Faculty of Science, Cairo University for statistical analyses, to Ali Abdelkhalek, Geology Department, Faculty of Science, Cairo University for assistance with the GPS analysis, and to Dr. Sameh Shaaban, Dr. Khalid Sayed and Dr. Rabab El-Haday (CAPL and PPRI of Agricultural Research Center, Giza, Egypt), and Hamdi AbdelMotalib for their help with whitefly field collections.

Funding Information:
Funding for this study was provided to AAM and EHS by The Research Support Fund Program of the Faculty of Science, Cairo University, Egypt. A grant from Cotton Incorporated to JKB, Project # 06–829, provided partial support to JRM, a Post-doctoral Associate at The University of Arizona, Tucson. Acknowledgements

Publisher Copyright:
© 2020, Springer Nature B.V.

ASJC Scopus Subject Areas

Plant Science
Insect Science

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10.1007/s12600-020-00858-9

Cite this

Metabolic resistance to organophosphate insecticides in natural populations of the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) in Egypt and molecular identification of mitotypes. / Shaurub, El Sayed H.; Paredes-Montero, Jorge R.; Brown, Judith K. et al.
In: Phytoparasitica, Vol. 49, No. 3, 07.2021, p. 443-457.

Research output: Contribution to journal › Article › peer-review

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title = "Metabolic resistance to organophosphate insecticides in natural populations of the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) in Egypt and molecular identification of mitotypes",

abstract = "The whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) cryptic species has become an important pest of cotton and vegetable crops in Egypt. In this study, resistance to three organophosphate insecticides (OPs), chlorpyrifos-methyl, profenofos and pirimiphos-methyl, and detoxification enzymatic activities of acetylcholinesterase, carboxylesterase, and monooxygenase were evaluated to establish baseline resistance levels for B. tabaci infesting cotton in seven Egyptian governorates, compared to a susceptible laboratory reference B. tabaci colony. Resistance to OPs ranged from low-to-moderate or low-to-high, while detoxification enzymatic activities were predominantly governorate-dependent. Phylogenetic analysis of a fragment of the mitochondria cytochrome oxidase I gene (756 bp) identified field-collected B. tabaci as the B mitotype. The results provide baseline OP resistance levels for B. tabaci that will aid in interpreting future monitoring outcomes and enable detection of insecticide resistance fluxes.",

author = "Shaurub, {El Sayed H.} and Paredes-Montero, {Jorge R.} and Brown, {Judith K.} and Zein, {Haggag S.} and Mohamed, {Amr A.}",

note = "Funding Information: The authors extend appreciation to Prof. Abdelhadi Abdallah, Department of Genetics, Faculty of Agriculture, Cairo University for providing whitefly colonies, to Dr. Mustafa M. Soliman, Department of Entomology, Faculty of Science, Cairo University for statistical analyses, to Ali Abdelkhalek, Geology Department, Faculty of Science, Cairo University for assistance with the GPS analysis, and to Dr. Sameh Shaaban, Dr. Khalid Sayed and Dr. Rabab El-Haday (CAPL and PPRI of Agricultural Research Center, Giza, Egypt), and Hamdi AbdelMotalib for their help with whitefly field collections. Funding Information: Funding for this study was provided to AAM and EHS by The Research Support Fund Program of the Faculty of Science, Cairo University, Egypt. A grant from Cotton Incorporated to JKB, Project # 06–829, provided partial support to JRM, a Post-doctoral Associate at The University of Arizona, Tucson. Acknowledgements Publisher Copyright: {\textcopyright} 2020, Springer Nature B.V.",

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Metabolic resistance to organophosphate insecticides in natural populations of the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) in Egypt and molecular identification of mitotypes

Abstract

Bibliographical note

ASJC Scopus Subject Areas

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