Nucleotide sequence of cDNA clones coding for a brain-type fatty acid binding protein and its tissue-specific expression in adult zebrafish (Danio rerio)

Eileen M. Denovan-Wright; Marianne Pierce; Jonathan M. Wright

doi:10.1016/S0167-4781(00)00075-0

Nucleotide sequence of cDNA clones coding for a brain-type fatty acid binding protein and its tissue-specific expression in adult zebrafish (Danio rerio)

Eileen M. Denovan-Wright, Marianne Pierce, Jonathan M. Wright

Medicine

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

We have determined the nucleotide sequence for two cDNA clones coding for a fatty acid binding protein (FABP) from zebrafish (Danio rerio). Comparison of the sequence with GenBank entries revealed extensive amino acid identity between this zebrafish FABP and brain FABPs (B-FABP) from other species. The zebrafish B-FABP cDNA hybridized to single restriction fragments of total zebrafish genomic DNA digested with the restriction endonucleases BglII or EcoRI suggesting that a single copy of the B-FABP gene is present in the zebrafish genome. Northern blot analysis demonstrated that the zebrafish B-FABP mRNA is approximately 850 nucleotides in length. In situ hybridization revealed that the B-FABP mRNA was expressed in the periventricular gray zone of the optic tectum of the adult zebrafish brain. Copyright (C) 2000 Elsevier Science B.V.

Original language	English
Pages (from-to)	221-226
Number of pages	6
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1492
Issue number	1
DOIs	https://doi.org/10.1016/S0167-4781(00)00075-0
Publication status	Published - Jun 21 2000

Bibliographical note

Funding Information:
This work was supported by a grant from the Natural Sciences and Engineering Research Council of Canada to J.M.W.

ASJC Scopus Subject Areas

Structural Biology
Biophysics
Biochemistry
Genetics

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Denovan-Wright, E. M., Pierce, M., & Wright, J. M. (2000). Nucleotide sequence of cDNA clones coding for a brain-type fatty acid binding protein and its tissue-specific expression in adult zebrafish (Danio rerio). Biochimica et Biophysica Acta - Gene Structure and Expression, 1492(1), 221-226. https://doi.org/10.1016/S0167-4781(00)00075-0

Nucleotide sequence of cDNA clones coding for a brain-type fatty acid binding protein and its tissue-specific expression in adult zebrafish (Danio rerio). / Denovan-Wright, Eileen M.; Pierce, Marianne; Wright, Jonathan M.
In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1492, No. 1, 21.06.2000, p. 221-226.

Research output: Contribution to journal › Article › peer-review

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Nucleotide sequence of cDNA clones coding for a brain-type fatty acid binding protein and its tissue-specific expression in adult zebrafish (Danio rerio)

Abstract

Bibliographical note

ASJC Scopus Subject Areas

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