Nucleotide sequence of cDNA clones coding for a brain-type fatty acid binding protein and its tissue-specific expression in adult zebrafish (Danio rerio)

Eileen M. Denovan-Wright; Marianne Pierce; Jonathan M. Wright

doi:10.1016/S0167-4781(00)00075-0

Nucleotide sequence of cDNA clones coding for a brain-type fatty acid binding protein and its tissue-specific expression in adult zebrafish (Danio rerio)

Eileen M. Denovan-Wright, Marianne Pierce, Jonathan M. Wright

Medicine

Résultat de recherche: Article › examen par les pairs

27 Citations (Scopus)

Résumé

We have determined the nucleotide sequence for two cDNA clones coding for a fatty acid binding protein (FABP) from zebrafish (Danio rerio). Comparison of the sequence with GenBank entries revealed extensive amino acid identity between this zebrafish FABP and brain FABPs (B-FABP) from other species. The zebrafish B-FABP cDNA hybridized to single restriction fragments of total zebrafish genomic DNA digested with the restriction endonucleases BglII or EcoRI suggesting that a single copy of the B-FABP gene is present in the zebrafish genome. Northern blot analysis demonstrated that the zebrafish B-FABP mRNA is approximately 850 nucleotides in length. In situ hybridization revealed that the B-FABP mRNA was expressed in the periventricular gray zone of the optic tectum of the adult zebrafish brain. Copyright (C) 2000 Elsevier Science B.V.

Langue d'origine	English
Pages (de-à)	221-226
Nombre de pages	6
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1492
Numéro de publication	1
DOI	https://doi.org/10.1016/S0167-4781(00)00075-0
Statut de publication	Published - juin 21 2000

Note bibliographique

Funding Information:
This work was supported by a grant from the Natural Sciences and Engineering Research Council of Canada to J.M.W.

ASJC Scopus Subject Areas

Structural Biology
Biophysics
Biochemistry
Genetics

Accès au document

10.1016/S0167-4781(00)00075-0

Autres fichiers et liens

Citer

Denovan-Wright, E. M., Pierce, M., & Wright, J. M. (2000). Nucleotide sequence of cDNA clones coding for a brain-type fatty acid binding protein and its tissue-specific expression in adult zebrafish (Danio rerio). Biochimica et Biophysica Acta - Gene Structure and Expression, 1492(1), 221-226. https://doi.org/10.1016/S0167-4781(00)00075-0

Nucleotide sequence of cDNA clones coding for a brain-type fatty acid binding protein and its tissue-specific expression in adult zebrafish (Danio rerio). / Denovan-Wright, Eileen M.; Pierce, Marianne; Wright, Jonathan M.
Dans: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1492, N° 1, 21.06.2000, p. 221-226.

Résultat de recherche: Article › examen par les pairs

@article{a3a53834dda745c68e99c30938731879,

title = "Nucleotide sequence of cDNA clones coding for a brain-type fatty acid binding protein and its tissue-specific expression in adult zebrafish (Danio rerio)",

abstract = "We have determined the nucleotide sequence for two cDNA clones coding for a fatty acid binding protein (FABP) from zebrafish (Danio rerio). Comparison of the sequence with GenBank entries revealed extensive amino acid identity between this zebrafish FABP and brain FABPs (B-FABP) from other species. The zebrafish B-FABP cDNA hybridized to single restriction fragments of total zebrafish genomic DNA digested with the restriction endonucleases BglII or EcoRI suggesting that a single copy of the B-FABP gene is present in the zebrafish genome. Northern blot analysis demonstrated that the zebrafish B-FABP mRNA is approximately 850 nucleotides in length. In situ hybridization revealed that the B-FABP mRNA was expressed in the periventricular gray zone of the optic tectum of the adult zebrafish brain. Copyright (C) 2000 Elsevier Science B.V.",

author = "Denovan-Wright, {Eileen M.} and Marianne Pierce and Wright, {Jonathan M.}",

note = "Funding Information: This work was supported by a grant from the Natural Sciences and Engineering Research Council of Canada to J.M.W. ",

year = "2000",

month = jun,

day = "21",

doi = "10.1016/S0167-4781(00)00075-0",

language = "English",

volume = "1492",

pages = "221--226",

journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",

issn = "0167-4781",

number = "1",

}

TY - JOUR

T1 - Nucleotide sequence of cDNA clones coding for a brain-type fatty acid binding protein and its tissue-specific expression in adult zebrafish (Danio rerio)

AU - Denovan-Wright, Eileen M.

AU - Pierce, Marianne

AU - Wright, Jonathan M.

N1 - Funding Information: This work was supported by a grant from the Natural Sciences and Engineering Research Council of Canada to J.M.W.

PY - 2000/6/21

Y1 - 2000/6/21

N2 - We have determined the nucleotide sequence for two cDNA clones coding for a fatty acid binding protein (FABP) from zebrafish (Danio rerio). Comparison of the sequence with GenBank entries revealed extensive amino acid identity between this zebrafish FABP and brain FABPs (B-FABP) from other species. The zebrafish B-FABP cDNA hybridized to single restriction fragments of total zebrafish genomic DNA digested with the restriction endonucleases BglII or EcoRI suggesting that a single copy of the B-FABP gene is present in the zebrafish genome. Northern blot analysis demonstrated that the zebrafish B-FABP mRNA is approximately 850 nucleotides in length. In situ hybridization revealed that the B-FABP mRNA was expressed in the periventricular gray zone of the optic tectum of the adult zebrafish brain. Copyright (C) 2000 Elsevier Science B.V.

AB - We have determined the nucleotide sequence for two cDNA clones coding for a fatty acid binding protein (FABP) from zebrafish (Danio rerio). Comparison of the sequence with GenBank entries revealed extensive amino acid identity between this zebrafish FABP and brain FABPs (B-FABP) from other species. The zebrafish B-FABP cDNA hybridized to single restriction fragments of total zebrafish genomic DNA digested with the restriction endonucleases BglII or EcoRI suggesting that a single copy of the B-FABP gene is present in the zebrafish genome. Northern blot analysis demonstrated that the zebrafish B-FABP mRNA is approximately 850 nucleotides in length. In situ hybridization revealed that the B-FABP mRNA was expressed in the periventricular gray zone of the optic tectum of the adult zebrafish brain. Copyright (C) 2000 Elsevier Science B.V.

UR - http://www.scopus.com/inward/record.url?scp=0034697736&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034697736&partnerID=8YFLogxK

U2 - 10.1016/S0167-4781(00)00075-0

DO - 10.1016/S0167-4781(00)00075-0

M3 - Article

C2 - 11004493

AN - SCOPUS:0034697736

SN - 0167-4781

VL - 1492

SP - 221

EP - 226

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

IS - 1

ER -

Nucleotide sequence of cDNA clones coding for a brain-type fatty acid binding protein and its tissue-specific expression in adult zebrafish (Danio rerio)

Résumé

Note bibliographique

ASJC Scopus Subject Areas

Accès au document

Autres fichiers et liens

Empreinte numérique

Citer