Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents

Simon Haile; Aidan M. Nikiforuk; Pawan K. Pandoh; David D.W. Twa; Duane E. Smailus; Jason Nguyen; Stephen Pleasance; Angus Wong; Yongjun Zhao; Diane Eisler; Michelle Moksa; Qi Cao; Marcus Wong; Edmund Su; Martin Krzywinski; Jessica Nelson; Andrew J. Mungall; Frankie Tsang; Leah M. Prentice; Agatha Jassem; Amee R. Manges; Steven J.M. Jones; Robin J. Coope; Natalie Prystajecky; Marco A. Marra; Mel Krajden; Martin Hirst

doi:10.1016/j.jviromet.2021.114339

Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents

Simon Haile, Aidan M. Nikiforuk, Pawan K. Pandoh, David D.W. Twa, Duane E. Smailus, Jason Nguyen, Stephen Pleasance, Angus Wong, Yongjun Zhao, Diane Eisler, Michelle Moksa, Qi Cao, Marcus Wong, Edmund Su, Martin Krzywinski, Jessica Nelson, Andrew J. Mungall, Frankie Tsang, Leah M. Prentice, Agatha JassemAmee R. Manges, Steven J.M. Jones, Robin J. Coope, Natalie Prystajecky, Marco A. Marra, Mel Krajden, Martin Hirst

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The COVID-19 pandemic has highlighted the need for generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers are readily amenable to meet this need. Here, one such approach is rigorously optimized to minimize cross-well contamination while maintaining sensitivity.

Original language	English
Article number	114339
Journal	Journal of Virological Methods
Volume	299
DOIs	https://doi.org/10.1016/j.jviromet.2021.114339
Publication status	Published - Jan 2022
Externally published	Yes

Bibliographical note

Funding Information:
Support for this work was provided by contributions from the Provincial Health Services Authority, Genome BC/Genome Canada ( 202SEQ , 212SEQ , 12002 , COV-088 ), the Canada Foundation for Innovation ( 20070 , 30981 , 30198 ) and the BC Knowledge Development Fund .

Publisher Copyright:
© 2021

ASJC Scopus Subject Areas

Virology

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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10.1016/j.jviromet.2021.114339

Cite this

Haile, S., Nikiforuk, A. M., Pandoh, P. K., Twa, D. D. W., Smailus, D. E., Nguyen, J., Pleasance, S., Wong, A., Zhao, Y., Eisler, D., Moksa, M., Cao, Q., Wong, M., Su, E., Krzywinski, M., Nelson, J., Mungall, A. J., Tsang, F., Prentice, L. M., ... Hirst, M. (2022). Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents. Journal of Virological Methods, 299, Article 114339. https://doi.org/10.1016/j.jviromet.2021.114339

Haile, S, Nikiforuk, AM, Pandoh, PK, Twa, DDW, Smailus, DE, Nguyen, J, Pleasance, S, Wong, A, Zhao, Y, Eisler, D, Moksa, M, Cao, Q, Wong, M, Su, E, Krzywinski, M, Nelson, J, Mungall, AJ, Tsang, F, Prentice, LM, Jassem, A, Manges, AR, Jones, SJM, Coope, RJ, Prystajecky, N, Marra, MA, Krajden, M & Hirst, M 2022, 'Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents', Journal of Virological Methods, vol. 299, 114339. https://doi.org/10.1016/j.jviromet.2021.114339

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title = "Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents",

abstract = "The COVID-19 pandemic has highlighted the need for generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers are readily amenable to meet this need. Here, one such approach is rigorously optimized to minimize cross-well contamination while maintaining sensitivity.",

author = "Simon Haile and Nikiforuk, {Aidan M.} and Pandoh, {Pawan K.} and Twa, {David D.W.} and Smailus, {Duane E.} and Jason Nguyen and Stephen Pleasance and Angus Wong and Yongjun Zhao and Diane Eisler and Michelle Moksa and Qi Cao and Marcus Wong and Edmund Su and Martin Krzywinski and Jessica Nelson and Mungall, {Andrew J.} and Frankie Tsang and Prentice, {Leah M.} and Agatha Jassem and Manges, {Amee R.} and Jones, {Steven J.M.} and Coope, {Robin J.} and Natalie Prystajecky and Marra, {Marco A.} and Mel Krajden and Martin Hirst",

note = "Funding Information: Support for this work was provided by contributions from the Provincial Health Services Authority, Genome BC/Genome Canada ( 202SEQ , 212SEQ , 12002 , COV-088 ), the Canada Foundation for Innovation ( 20070 , 30981 , 30198 ) and the BC Knowledge Development Fund . Publisher Copyright: {\textcopyright} 2021",

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doi = "10.1016/j.jviromet.2021.114339",

language = "English",

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AU - Haile, Simon

AU - Nikiforuk, Aidan M.

AU - Pandoh, Pawan K.

AU - Twa, David D.W.

AU - Smailus, Duane E.

AU - Nguyen, Jason

AU - Pleasance, Stephen

AU - Wong, Angus

AU - Zhao, Yongjun

AU - Eisler, Diane

AU - Moksa, Michelle

AU - Cao, Qi

AU - Wong, Marcus

AU - Su, Edmund

AU - Krzywinski, Martin

AU - Nelson, Jessica

AU - Mungall, Andrew J.

AU - Tsang, Frankie

AU - Prentice, Leah M.

AU - Jassem, Agatha

AU - Manges, Amee R.

AU - Jones, Steven J.M.

AU - Coope, Robin J.

AU - Prystajecky, Natalie

AU - Marra, Marco A.

AU - Krajden, Mel

AU - Hirst, Martin

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PY - 2022/1

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Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents

Abstract

Bibliographical note

ASJC Scopus Subject Areas

PubMed: MeSH publication types

Access to Document

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