Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents

Simon Haile; Aidan M. Nikiforuk; Pawan K. Pandoh; David D.W. Twa; Duane E. Smailus; Jason Nguyen; Stephen Pleasance; Angus Wong; Yongjun Zhao; Diane Eisler; Michelle Moksa; Qi Cao; Marcus Wong; Edmund Su; Martin Krzywinski; Jessica Nelson; Andrew J. Mungall; Frankie Tsang; Leah M. Prentice; Agatha Jassem; Amee R. Manges; Steven J.M. Jones; Robin J. Coope; Natalie Prystajecky; Marco A. Marra; Mel Krajden; Martin Hirst

doi:10.1016/j.jviromet.2021.114339

Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents

Simon Haile, Aidan M. Nikiforuk, Pawan K. Pandoh, David D.W. Twa, Duane E. Smailus, Jason Nguyen, Stephen Pleasance, Angus Wong, Yongjun Zhao, Diane Eisler, Michelle Moksa, Qi Cao, Marcus Wong, Edmund Su, Martin Krzywinski, Jessica Nelson, Andrew J. Mungall, Frankie Tsang, Leah M. Prentice, Agatha JassemAmee R. Manges, Steven J.M. Jones, Robin J. Coope, Natalie Prystajecky, Marco A. Marra, Mel Krajden, Martin Hirst

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

6 Citas (Scopus)

Resumen

The COVID-19 pandemic has highlighted the need for generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers are readily amenable to meet this need. Here, one such approach is rigorously optimized to minimize cross-well contamination while maintaining sensitivity.

Idioma original	English
Número de artículo	114339
Publicación	Journal of Virological Methods
Volumen	299
DOI	https://doi.org/10.1016/j.jviromet.2021.114339
Estado	Published - ene. 2022
Publicado de forma externa	Sí

Nota bibliográfica

Funding Information:
Support for this work was provided by contributions from the Provincial Health Services Authority, Genome BC/Genome Canada ( 202SEQ , 212SEQ , 12002 , COV-088 ), the Canada Foundation for Innovation ( 20070 , 30981 , 30198 ) and the BC Knowledge Development Fund .

Publisher Copyright:
© 2021

ASJC Scopus Subject Areas

Virology

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

Acceder al documento

10.1016/j.jviromet.2021.114339

Otros archivos y enlaces

Citar esto

Haile, S., Nikiforuk, A. M., Pandoh, P. K., Twa, D. D. W., Smailus, D. E., Nguyen, J., Pleasance, S., Wong, A., Zhao, Y., Eisler, D., Moksa, M., Cao, Q., Wong, M., Su, E., Krzywinski, M., Nelson, J., Mungall, A. J., Tsang, F., Prentice, L. M., ... Hirst, M. (2022). Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents. Journal of Virological Methods, 299, Artículo 114339. https://doi.org/10.1016/j.jviromet.2021.114339

Haile, S, Nikiforuk, AM, Pandoh, PK, Twa, DDW, Smailus, DE, Nguyen, J, Pleasance, S, Wong, A, Zhao, Y, Eisler, D, Moksa, M, Cao, Q, Wong, M, Su, E, Krzywinski, M, Nelson, J, Mungall, AJ, Tsang, F, Prentice, LM, Jassem, A, Manges, AR, Jones, SJM, Coope, RJ, Prystajecky, N, Marra, MA, Krajden, M & Hirst, M 2022, 'Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents', Journal of Virological Methods, vol. 299, 114339. https://doi.org/10.1016/j.jviromet.2021.114339

@article{a4b4b5fb991b4932a5fe5edb03ab38e4,

title = "Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents",

abstract = "The COVID-19 pandemic has highlighted the need for generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers are readily amenable to meet this need. Here, one such approach is rigorously optimized to minimize cross-well contamination while maintaining sensitivity.",

author = "Simon Haile and Nikiforuk, {Aidan M.} and Pandoh, {Pawan K.} and Twa, {David D.W.} and Smailus, {Duane E.} and Jason Nguyen and Stephen Pleasance and Angus Wong and Yongjun Zhao and Diane Eisler and Michelle Moksa and Qi Cao and Marcus Wong and Edmund Su and Martin Krzywinski and Jessica Nelson and Mungall, {Andrew J.} and Frankie Tsang and Prentice, {Leah M.} and Agatha Jassem and Manges, {Amee R.} and Jones, {Steven J.M.} and Coope, {Robin J.} and Natalie Prystajecky and Marra, {Marco A.} and Mel Krajden and Martin Hirst",

note = "Funding Information: Support for this work was provided by contributions from the Provincial Health Services Authority, Genome BC/Genome Canada ( 202SEQ , 212SEQ , 12002 , COV-088 ), the Canada Foundation for Innovation ( 20070 , 30981 , 30198 ) and the BC Knowledge Development Fund . Publisher Copyright: {\textcopyright} 2021",

year = "2022",

month = jan,

doi = "10.1016/j.jviromet.2021.114339",

language = "English",

volume = "299",

journal = "Journal of Virological Methods",

issn = "0166-0934",

publisher = "Elsevier",

}

TY - JOUR

T1 - Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents

AU - Haile, Simon

AU - Nikiforuk, Aidan M.

AU - Pandoh, Pawan K.

AU - Twa, David D.W.

AU - Smailus, Duane E.

AU - Nguyen, Jason

AU - Pleasance, Stephen

AU - Wong, Angus

AU - Zhao, Yongjun

AU - Eisler, Diane

AU - Moksa, Michelle

AU - Cao, Qi

AU - Wong, Marcus

AU - Su, Edmund

AU - Krzywinski, Martin

AU - Nelson, Jessica

AU - Mungall, Andrew J.

AU - Tsang, Frankie

AU - Prentice, Leah M.

AU - Jassem, Agatha

AU - Manges, Amee R.

AU - Jones, Steven J.M.

AU - Coope, Robin J.

AU - Prystajecky, Natalie

AU - Marra, Marco A.

AU - Krajden, Mel

AU - Hirst, Martin

N1 - Funding Information: Support for this work was provided by contributions from the Provincial Health Services Authority, Genome BC/Genome Canada ( 202SEQ , 212SEQ , 12002 , COV-088 ), the Canada Foundation for Innovation ( 20070 , 30981 , 30198 ) and the BC Knowledge Development Fund . Publisher Copyright: © 2021

PY - 2022/1

Y1 - 2022/1

N2 - The COVID-19 pandemic has highlighted the need for generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers are readily amenable to meet this need. Here, one such approach is rigorously optimized to minimize cross-well contamination while maintaining sensitivity.

AB - The COVID-19 pandemic has highlighted the need for generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers are readily amenable to meet this need. Here, one such approach is rigorously optimized to minimize cross-well contamination while maintaining sensitivity.

UR - http://www.scopus.com/inward/record.url?scp=85117892260&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85117892260&partnerID=8YFLogxK

U2 - 10.1016/j.jviromet.2021.114339

DO - 10.1016/j.jviromet.2021.114339

M3 - Article

C2 - 34687784

AN - SCOPUS:85117892260

SN - 0166-0934

VL - 299

JO - Journal of Virological Methods

JF - Journal of Virological Methods

M1 - 114339

ER -

Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents

Resumen

Nota bibliográfica

ASJC Scopus Subject Areas

PubMed: MeSH publication types

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto