Orthovanadate inhibits adrenaline-induced hyperpolarization in amphibian sympathetic ganglia

P. E. Rafuse; P. A. Smith; R. J. Walker

doi:10.1016/0028-3908(88)90204-3

Orthovanadate inhibits adrenaline-induced hyperpolarization in amphibian sympathetic ganglia

P. E. Rafuse, P. A. Smith, R. J. Walker

Research output: Contribution to journal › Article › peer-review

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Abstract

The adrenaline-induced hyperpolarization of neurones in sympathetic ganglia of Rana pipiens was recorded by means of the sucrose gap technique. This hyperpolarization was inhibited in a concentration-dependent manner by sodium orthovanadate (0.1-l.0mM). Although orthovanadate inhibited the hydrolysis of ATP by preparations of microsomal Na⁺/K⁺ ATPase from frog nerve (IC₅₀ = 3.0 μ M), extracellulariy-applied orthovanadate did not inhibit electrogenic sodium pumping in intact sympathetic ganglia of Rana pipiens. This and other observations indicated that extracellularly-applied orthovanadate did not enter sympathetic neurones and did not have access to intracellular enzyme systems. It is suggested that orthovanadate acts at an extracellular site to inhibit the adrenaline-induced hyperpolarization.

Original language	English
Pages (from-to)	79-83
Number of pages	5
Journal	Neuropharmacology
Volume	27
Issue number	1
DOIs	https://doi.org/10.1016/0028-3908(88)90204-3
Publication status	Published - Jan 1988
Externally published	Yes

Bibliographical note

Funding Information:
Aek~5w~e~gemenrs-Suppobrtye gdr antsf rom the Alberta Mental Health Advisory Council and Alberta Heritage Foundation for Medical Research.R . J. W. gratefully acknowledgeas travelg rantf rom the N&held Foundation. We thank Drs A. F. Almeida and R. Reithmierf or useful discussions.

ASJC Scopus Subject Areas

Pharmacology
Cellular and Molecular Neuroscience

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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10.1016/0028-3908(88)90204-3

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@article{d52e7b3ef9734e12bb8ba2350394cc62,

title = "Orthovanadate inhibits adrenaline-induced hyperpolarization in amphibian sympathetic ganglia",

abstract = "The adrenaline-induced hyperpolarization of neurones in sympathetic ganglia of Rana pipiens was recorded by means of the sucrose gap technique. This hyperpolarization was inhibited in a concentration-dependent manner by sodium orthovanadate (0.1-l.0mM). Although orthovanadate inhibited the hydrolysis of ATP by preparations of microsomal Na+/K+ ATPase from frog nerve (IC50 = 3.0 μ M), extracellulariy-applied orthovanadate did not inhibit electrogenic sodium pumping in intact sympathetic ganglia of Rana pipiens. This and other observations indicated that extracellularly-applied orthovanadate did not enter sympathetic neurones and did not have access to intracellular enzyme systems. It is suggested that orthovanadate acts at an extracellular site to inhibit the adrenaline-induced hyperpolarization.",

author = "Rafuse, {P. E.} and Smith, {P. A.} and Walker, {R. J.}",

note = "Funding Information: Aek~5w~e~gemenrs-Suppobrtye gdr antsf rom the Alberta Mental Health Advisory Council and Alberta Heritage Foundation for Medical Research.R . J. W. gratefully acknowledgeas travelg rantf rom the N&held Foundation. We thank Drs A. F. Almeida and R. Reithmierf or useful discussions.",

year = "1988",

month = jan,

doi = "10.1016/0028-3908(88)90204-3",

language = "English",

volume = "27",

pages = "79--83",

journal = "Neuropharmacology",

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T1 - Orthovanadate inhibits adrenaline-induced hyperpolarization in amphibian sympathetic ganglia

AU - Rafuse, P. E.

AU - Smith, P. A.

AU - Walker, R. J.

N1 - Funding Information: Aek~5w~e~gemenrs-Suppobrtye gdr antsf rom the Alberta Mental Health Advisory Council and Alberta Heritage Foundation for Medical Research.R . J. W. gratefully acknowledgeas travelg rantf rom the N&held Foundation. We thank Drs A. F. Almeida and R. Reithmierf or useful discussions.

PY - 1988/1

Y1 - 1988/1

N2 - The adrenaline-induced hyperpolarization of neurones in sympathetic ganglia of Rana pipiens was recorded by means of the sucrose gap technique. This hyperpolarization was inhibited in a concentration-dependent manner by sodium orthovanadate (0.1-l.0mM). Although orthovanadate inhibited the hydrolysis of ATP by preparations of microsomal Na+/K+ ATPase from frog nerve (IC50 = 3.0 μ M), extracellulariy-applied orthovanadate did not inhibit electrogenic sodium pumping in intact sympathetic ganglia of Rana pipiens. This and other observations indicated that extracellularly-applied orthovanadate did not enter sympathetic neurones and did not have access to intracellular enzyme systems. It is suggested that orthovanadate acts at an extracellular site to inhibit the adrenaline-induced hyperpolarization.

AB - The adrenaline-induced hyperpolarization of neurones in sympathetic ganglia of Rana pipiens was recorded by means of the sucrose gap technique. This hyperpolarization was inhibited in a concentration-dependent manner by sodium orthovanadate (0.1-l.0mM). Although orthovanadate inhibited the hydrolysis of ATP by preparations of microsomal Na+/K+ ATPase from frog nerve (IC50 = 3.0 μ M), extracellulariy-applied orthovanadate did not inhibit electrogenic sodium pumping in intact sympathetic ganglia of Rana pipiens. This and other observations indicated that extracellularly-applied orthovanadate did not enter sympathetic neurones and did not have access to intracellular enzyme systems. It is suggested that orthovanadate acts at an extracellular site to inhibit the adrenaline-induced hyperpolarization.

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VL - 27

SP - 79

EP - 83

JO - Neuropharmacology

JF - Neuropharmacology

IS - 1

ER -

Orthovanadate inhibits adrenaline-induced hyperpolarization in amphibian sympathetic ganglia

Abstract

Bibliographical note

ASJC Scopus Subject Areas

PubMed: MeSH publication types

Access to Document

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