Orthovanadate inhibits adrenaline-induced hyperpolarization in amphibian sympathetic ganglia

P. E. Rafuse; P. A. Smith; R. J. Walker

doi:10.1016/0028-3908(88)90204-3

Orthovanadate inhibits adrenaline-induced hyperpolarization in amphibian sympathetic ganglia

P. E. Rafuse, P. A. Smith, R. J. Walker

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Resumen

The adrenaline-induced hyperpolarization of neurones in sympathetic ganglia of Rana pipiens was recorded by means of the sucrose gap technique. This hyperpolarization was inhibited in a concentration-dependent manner by sodium orthovanadate (0.1-l.0mM). Although orthovanadate inhibited the hydrolysis of ATP by preparations of microsomal Na⁺/K⁺ ATPase from frog nerve (IC₅₀ = 3.0 μ M), extracellulariy-applied orthovanadate did not inhibit electrogenic sodium pumping in intact sympathetic ganglia of Rana pipiens. This and other observations indicated that extracellularly-applied orthovanadate did not enter sympathetic neurones and did not have access to intracellular enzyme systems. It is suggested that orthovanadate acts at an extracellular site to inhibit the adrenaline-induced hyperpolarization.

Idioma original	English
Páginas (desde-hasta)	79-83
Número de páginas	5
Publicación	Neuropharmacology
Volumen	27
N.º	1
DOI	https://doi.org/10.1016/0028-3908(88)90204-3
Estado	Published - ene. 1988
Publicado de forma externa	Sí

Nota bibliográfica

Funding Information:
Aek~5w~e~gemenrs-Suppobrtye gdr antsf rom the Alberta Mental Health Advisory Council and Alberta Heritage Foundation for Medical Research.R . J. W. gratefully acknowledgeas travelg rantf rom the N&held Foundation. We thank Drs A. F. Almeida and R. Reithmierf or useful discussions.

ASJC Scopus Subject Areas

Pharmacology
Cellular and Molecular Neuroscience

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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10.1016/0028-3908(88)90204-3

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@article{d52e7b3ef9734e12bb8ba2350394cc62,

title = "Orthovanadate inhibits adrenaline-induced hyperpolarization in amphibian sympathetic ganglia",

abstract = "The adrenaline-induced hyperpolarization of neurones in sympathetic ganglia of Rana pipiens was recorded by means of the sucrose gap technique. This hyperpolarization was inhibited in a concentration-dependent manner by sodium orthovanadate (0.1-l.0mM). Although orthovanadate inhibited the hydrolysis of ATP by preparations of microsomal Na+/K+ ATPase from frog nerve (IC50 = 3.0 μ M), extracellulariy-applied orthovanadate did not inhibit electrogenic sodium pumping in intact sympathetic ganglia of Rana pipiens. This and other observations indicated that extracellularly-applied orthovanadate did not enter sympathetic neurones and did not have access to intracellular enzyme systems. It is suggested that orthovanadate acts at an extracellular site to inhibit the adrenaline-induced hyperpolarization.",

author = "Rafuse, {P. E.} and Smith, {P. A.} and Walker, {R. J.}",

note = "Funding Information: Aek~5w~e~gemenrs-Suppobrtye gdr antsf rom the Alberta Mental Health Advisory Council and Alberta Heritage Foundation for Medical Research.R . J. W. gratefully acknowledgeas travelg rantf rom the N&held Foundation. We thank Drs A. F. Almeida and R. Reithmierf or useful discussions.",

year = "1988",

month = jan,

doi = "10.1016/0028-3908(88)90204-3",

language = "English",

volume = "27",

pages = "79--83",

journal = "Neuropharmacology",

issn = "0028-3908",

publisher = "Elsevier Limited",

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T1 - Orthovanadate inhibits adrenaline-induced hyperpolarization in amphibian sympathetic ganglia

AU - Rafuse, P. E.

AU - Smith, P. A.

AU - Walker, R. J.

N1 - Funding Information: Aek~5w~e~gemenrs-Suppobrtye gdr antsf rom the Alberta Mental Health Advisory Council and Alberta Heritage Foundation for Medical Research.R . J. W. gratefully acknowledgeas travelg rantf rom the N&held Foundation. We thank Drs A. F. Almeida and R. Reithmierf or useful discussions.

PY - 1988/1

Y1 - 1988/1

N2 - The adrenaline-induced hyperpolarization of neurones in sympathetic ganglia of Rana pipiens was recorded by means of the sucrose gap technique. This hyperpolarization was inhibited in a concentration-dependent manner by sodium orthovanadate (0.1-l.0mM). Although orthovanadate inhibited the hydrolysis of ATP by preparations of microsomal Na+/K+ ATPase from frog nerve (IC50 = 3.0 μ M), extracellulariy-applied orthovanadate did not inhibit electrogenic sodium pumping in intact sympathetic ganglia of Rana pipiens. This and other observations indicated that extracellularly-applied orthovanadate did not enter sympathetic neurones and did not have access to intracellular enzyme systems. It is suggested that orthovanadate acts at an extracellular site to inhibit the adrenaline-induced hyperpolarization.

AB - The adrenaline-induced hyperpolarization of neurones in sympathetic ganglia of Rana pipiens was recorded by means of the sucrose gap technique. This hyperpolarization was inhibited in a concentration-dependent manner by sodium orthovanadate (0.1-l.0mM). Although orthovanadate inhibited the hydrolysis of ATP by preparations of microsomal Na+/K+ ATPase from frog nerve (IC50 = 3.0 μ M), extracellulariy-applied orthovanadate did not inhibit electrogenic sodium pumping in intact sympathetic ganglia of Rana pipiens. This and other observations indicated that extracellularly-applied orthovanadate did not enter sympathetic neurones and did not have access to intracellular enzyme systems. It is suggested that orthovanadate acts at an extracellular site to inhibit the adrenaline-induced hyperpolarization.

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U2 - 10.1016/0028-3908(88)90204-3

DO - 10.1016/0028-3908(88)90204-3

M3 - Article

C2 - 2832779

AN - SCOPUS:0023745546

SN - 0028-3908

VL - 27

SP - 79

EP - 83

JO - Neuropharmacology

JF - Neuropharmacology

IS - 1

ER -

Orthovanadate inhibits adrenaline-induced hyperpolarization in amphibian sympathetic ganglia

Resumen

Nota bibliográfica

ASJC Scopus Subject Areas

PubMed: MeSH publication types

Acceder al documento

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Huella

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