Processing a 16S rRNA Sequencing Dataset with the Microbiome Helper Workflow

Gavin M. Douglas; André M. Comeau; Morgan G.I. Langille

doi:10.1007/978-1-4939-8728-3_9

Processing a 16S rRNA Sequencing Dataset with the Microbiome Helper Workflow

Gavin M. Douglas, André M. Comeau, Morgan G.I. Langille

Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter

11 Citations (Scopus)

Abstract

Sequencing microbiome samples has recently become a fast and cost-effective method to taxonomically profile communities. The growing interest in analyzing microbial sequencing data has attracted many new researchers to the field. Here, we present a straightforward bioinformatic pipeline that aims to streamline the processing of 16S rRNA sequencing data. This workflow is part of the larger project called Microbiome Helper (Comeau et al. mSyst 2:e00127-16, 2017), which includes other bioinformatic workflows, tutorials, and scripts available here: https://github.com/mlangill/microbiome_helper/wiki.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	131-141
Number of pages	11
DOIs	https://doi.org/10.1007/978-1-4939-8728-3_9
Publication status	Published - 2018

Publication series

Name	Methods in Molecular Biology
Volume	1849
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© 2018, Springer Science+Business Media, LLC, part of Springer Nature.

ASJC Scopus Subject Areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-8728-3_9

Cite this

@inbook{98aaa8243edf4214b0fa7e10c2464c8e,

title = "Processing a 16S rRNA Sequencing Dataset with the Microbiome Helper Workflow",

abstract = "Sequencing microbiome samples has recently become a fast and cost-effective method to taxonomically profile communities. The growing interest in analyzing microbial sequencing data has attracted many new researchers to the field. Here, we present a straightforward bioinformatic pipeline that aims to streamline the processing of 16S rRNA sequencing data. This workflow is part of the larger project called Microbiome Helper (Comeau et al. mSyst 2:e00127-16, 2017), which includes other bioinformatic workflows, tutorials, and scripts available here: https://github.com/mlangill/microbiome_helper/wiki.",

author = "Douglas, {Gavin M.} and Comeau, {Andr{\'e} M.} and Langille, {Morgan G.I.}",

note = "Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2018",

doi = "10.1007/978-1-4939-8728-3_9",

language = "English",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc.",

pages = "131--141",

booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Processing a 16S rRNA Sequencing Dataset with the Microbiome Helper Workflow

AU - Douglas, Gavin M.

AU - Comeau, André M.

AU - Langille, Morgan G.I.

PY - 2018

Y1 - 2018

N2 - Sequencing microbiome samples has recently become a fast and cost-effective method to taxonomically profile communities. The growing interest in analyzing microbial sequencing data has attracted many new researchers to the field. Here, we present a straightforward bioinformatic pipeline that aims to streamline the processing of 16S rRNA sequencing data. This workflow is part of the larger project called Microbiome Helper (Comeau et al. mSyst 2:e00127-16, 2017), which includes other bioinformatic workflows, tutorials, and scripts available here: https://github.com/mlangill/microbiome_helper/wiki.

AB - Sequencing microbiome samples has recently become a fast and cost-effective method to taxonomically profile communities. The growing interest in analyzing microbial sequencing data has attracted many new researchers to the field. Here, we present a straightforward bioinformatic pipeline that aims to streamline the processing of 16S rRNA sequencing data. This workflow is part of the larger project called Microbiome Helper (Comeau et al. mSyst 2:e00127-16, 2017), which includes other bioinformatic workflows, tutorials, and scripts available here: https://github.com/mlangill/microbiome_helper/wiki.

UR - http://www.scopus.com/inward/record.url?scp=85054646587&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85054646587&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-8728-3_9

DO - 10.1007/978-1-4939-8728-3_9

M3 - Chapter

C2 - 30298252

AN - SCOPUS:85054646587

T3 - Methods in Molecular Biology

SP - 131

EP - 141

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Processing a 16S rRNA Sequencing Dataset with the Microbiome Helper Workflow

Abstract

Publication series

Bibliographical note

ASJC Scopus Subject Areas

Access to Document

Other files and links

Fingerprint

Cite this