T lymphocytes mediate leaflet destruction and allograft aortic valve failure in rats

Jean François Legare; Tim D.G. Lee; Kimberley Creaser; David B. Ross; Marjorie Green

doi:10.1016/S0003-4975(00)01677-5

T lymphocytes mediate leaflet destruction and allograft aortic valve failure in rats

Jean François Legare, Tim D.G. Lee, Kimberley Creaser, David B. Ross, Marjorie Green

Medicine

Medicine

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

Background: Allograft heart valves are commonly used in cardiac surgery but ultimately fail. This situation is most acute in children. This study addresses the role of T cell-mediated immune damage in allograft valve failure. Methods: Syngeneic (Lewis to Lewis) or allogeneic (Brown Norway to Lewis) aortic valve grafts were implanted infrarenally into Lewis rat recipients (n = 24). Allogeneic valve grafts were also implanted into T cell-deficient rats (nude; n = 12). At 7, 14, and 28 days the valves were explanted and examined for structural integrity and cellular infiltration. Results: Syngeneic grafts maintained normal leaflet structure with little leaflet immune infiltration. Allografts showed leaflet infiltration (7 days), significant leaflet thickening, progressively decreased cellularity (14 days), and leaflet destruction (28 days). Infiltrates contained CD43+, CD3+, and CDS+ cells. Allografts in T cell-deficient rats showed none of the above changes and maintained normal structural integrity. Conclusions: Allograft heart valves in the rat model undergo T cell-mediated immune rejection, resulting in structural failure. (C) 2000 by The Society of Thoracic Surgeons.

Original language	English
Pages (from-to)	1238-1245
Number of pages	8
Journal	Annals of Thoracic Surgery
Volume	70
Issue number	4
DOIs	https://doi.org/10.1016/S0003-4975(00)01677-5
Publication status	Published - 2000

Bibliographical note

Funding Information:
This work was supported by The Toronto Hospital for Sick Children Research Foundation.

ASJC Scopus Subject Areas

Surgery
Pulmonary and Respiratory Medicine
Cardiology and Cardiovascular Medicine

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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10.1016/S0003-4975(00)01677-5

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title = "T lymphocytes mediate leaflet destruction and allograft aortic valve failure in rats",

abstract = "Background: Allograft heart valves are commonly used in cardiac surgery but ultimately fail. This situation is most acute in children. This study addresses the role of T cell-mediated immune damage in allograft valve failure. Methods: Syngeneic (Lewis to Lewis) or allogeneic (Brown Norway to Lewis) aortic valve grafts were implanted infrarenally into Lewis rat recipients (n = 24). Allogeneic valve grafts were also implanted into T cell-deficient rats (nude; n = 12). At 7, 14, and 28 days the valves were explanted and examined for structural integrity and cellular infiltration. Results: Syngeneic grafts maintained normal leaflet structure with little leaflet immune infiltration. Allografts showed leaflet infiltration (7 days), significant leaflet thickening, progressively decreased cellularity (14 days), and leaflet destruction (28 days). Infiltrates contained CD43+, CD3+, and CDS+ cells. Allografts in T cell-deficient rats showed none of the above changes and maintained normal structural integrity. Conclusions: Allograft heart valves in the rat model undergo T cell-mediated immune rejection, resulting in structural failure. (C) 2000 by The Society of Thoracic Surgeons.",

author = "Legare, {Jean Fran{\c c}ois} and Lee, {Tim D.G.} and Kimberley Creaser and Ross, {David B.} and Marjorie Green",

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doi = "10.1016/S0003-4975(00)01677-5",

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T1 - T lymphocytes mediate leaflet destruction and allograft aortic valve failure in rats

AU - Legare, Jean François

AU - Lee, Tim D.G.

AU - Creaser, Kimberley

AU - Ross, David B.

AU - Green, Marjorie

N1 - Funding Information: This work was supported by The Toronto Hospital for Sick Children Research Foundation.

PY - 2000

Y1 - 2000

N2 - Background: Allograft heart valves are commonly used in cardiac surgery but ultimately fail. This situation is most acute in children. This study addresses the role of T cell-mediated immune damage in allograft valve failure. Methods: Syngeneic (Lewis to Lewis) or allogeneic (Brown Norway to Lewis) aortic valve grafts were implanted infrarenally into Lewis rat recipients (n = 24). Allogeneic valve grafts were also implanted into T cell-deficient rats (nude; n = 12). At 7, 14, and 28 days the valves were explanted and examined for structural integrity and cellular infiltration. Results: Syngeneic grafts maintained normal leaflet structure with little leaflet immune infiltration. Allografts showed leaflet infiltration (7 days), significant leaflet thickening, progressively decreased cellularity (14 days), and leaflet destruction (28 days). Infiltrates contained CD43+, CD3+, and CDS+ cells. Allografts in T cell-deficient rats showed none of the above changes and maintained normal structural integrity. Conclusions: Allograft heart valves in the rat model undergo T cell-mediated immune rejection, resulting in structural failure. (C) 2000 by The Society of Thoracic Surgeons.

AB - Background: Allograft heart valves are commonly used in cardiac surgery but ultimately fail. This situation is most acute in children. This study addresses the role of T cell-mediated immune damage in allograft valve failure. Methods: Syngeneic (Lewis to Lewis) or allogeneic (Brown Norway to Lewis) aortic valve grafts were implanted infrarenally into Lewis rat recipients (n = 24). Allogeneic valve grafts were also implanted into T cell-deficient rats (nude; n = 12). At 7, 14, and 28 days the valves were explanted and examined for structural integrity and cellular infiltration. Results: Syngeneic grafts maintained normal leaflet structure with little leaflet immune infiltration. Allografts showed leaflet infiltration (7 days), significant leaflet thickening, progressively decreased cellularity (14 days), and leaflet destruction (28 days). Infiltrates contained CD43+, CD3+, and CDS+ cells. Allografts in T cell-deficient rats showed none of the above changes and maintained normal structural integrity. Conclusions: Allograft heart valves in the rat model undergo T cell-mediated immune rejection, resulting in structural failure. (C) 2000 by The Society of Thoracic Surgeons.

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T lymphocytes mediate leaflet destruction and allograft aortic valve failure in rats

Abstract

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