The partial purification of deoxynucleoside monophosphate kinases from L cells

Thomas J. Griffith; Christopher W. Helleiner

doi:10.1016/0005-2787(65)90113-9

The partial purification of deoxynucleoside monophosphate kinases from L cells

Thomas J. Griffith, Christopher W. Helleiner

Medicine

Medicine

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

1. 1. Sonically disrupted L cells contain enzymes catalyzing the formation of all four deoxynucleoside di- and triphosphates from the monophosphates. 2. 2. The deoxyguanylate and deoxyadenylate kinases, which catalyze the formation of the corresponding diphosphates have been partially purified, and have each been obtained free of other deoxynucleotide kinases and of deoxynucleosidediphosphate kinases. 3. 3. The separate identities of deoxycytidylate and thymidylate kinases are inferred from experiments in which these enzymes were selectively inactivated. 4. 4. Both of the purine deoxynucleotide kinases also catalyze the phosphorylation of the corresponding ribonucleotides. Other properties of these enzymes have been studied.

Original language	English
Pages (from-to)	114-124
Number of pages	11
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	108
Issue number	1
DOIs	https://doi.org/10.1016/0005-2787(65)90113-9
Publication status	Published - Sept 6 1965

Bibliographical note

Funding Information:
The authors wish to acknowledgeth e skilled technicala ssistanceo f Mrs. I. Oss, and the many helpful discussionws ith Dr. L. SI~INOVlTCH. Financial support was receivedfr omthe NationalC ancerI nstituteo f Canadaa nd the Medical Research Council of Canada.O ne of us (T.J.G.) was supportedb y the ManitobaC ancerT reat-ment and ResearchF oundation.

ASJC Scopus Subject Areas

General Medicine

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title = "The partial purification of deoxynucleoside monophosphate kinases from L cells",

abstract = "1. 1. Sonically disrupted L cells contain enzymes catalyzing the formation of all four deoxynucleoside di- and triphosphates from the monophosphates. 2. 2. The deoxyguanylate and deoxyadenylate kinases, which catalyze the formation of the corresponding diphosphates have been partially purified, and have each been obtained free of other deoxynucleotide kinases and of deoxynucleosidediphosphate kinases. 3. 3. The separate identities of deoxycytidylate and thymidylate kinases are inferred from experiments in which these enzymes were selectively inactivated. 4. 4. Both of the purine deoxynucleotide kinases also catalyze the phosphorylation of the corresponding ribonucleotides. Other properties of these enzymes have been studied.",

author = "Griffith, {Thomas J.} and Helleiner, {Christopher W.}",

note = "Funding Information: The authors wish to acknowledgeth e skilled technicala ssistanceo f Mrs. I. Oss, and the many helpful discussionws ith Dr. L. SI~INOVlTCH. Financial support was receivedfr omthe NationalC ancerI nstituteo f Canadaa nd the Medical Research Council of Canada.O ne of us (T.J.G.) was supportedb y the ManitobaC ancerT reat-ment and ResearchF oundation.",

year = "1965",

month = sep,

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doi = "10.1016/0005-2787(65)90113-9",

language = "English",

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journal = "BBA Section Nucleic Acids And Protein Synthesis",

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T1 - The partial purification of deoxynucleoside monophosphate kinases from L cells

AU - Griffith, Thomas J.

AU - Helleiner, Christopher W.

N1 - Funding Information: The authors wish to acknowledgeth e skilled technicala ssistanceo f Mrs. I. Oss, and the many helpful discussionws ith Dr. L. SI~INOVlTCH. Financial support was receivedfr omthe NationalC ancerI nstituteo f Canadaa nd the Medical Research Council of Canada.O ne of us (T.J.G.) was supportedb y the ManitobaC ancerT reat-ment and ResearchF oundation.

PY - 1965/9/6

Y1 - 1965/9/6

N2 - 1. 1. Sonically disrupted L cells contain enzymes catalyzing the formation of all four deoxynucleoside di- and triphosphates from the monophosphates. 2. 2. The deoxyguanylate and deoxyadenylate kinases, which catalyze the formation of the corresponding diphosphates have been partially purified, and have each been obtained free of other deoxynucleotide kinases and of deoxynucleosidediphosphate kinases. 3. 3. The separate identities of deoxycytidylate and thymidylate kinases are inferred from experiments in which these enzymes were selectively inactivated. 4. 4. Both of the purine deoxynucleotide kinases also catalyze the phosphorylation of the corresponding ribonucleotides. Other properties of these enzymes have been studied.

AB - 1. 1. Sonically disrupted L cells contain enzymes catalyzing the formation of all four deoxynucleoside di- and triphosphates from the monophosphates. 2. 2. The deoxyguanylate and deoxyadenylate kinases, which catalyze the formation of the corresponding diphosphates have been partially purified, and have each been obtained free of other deoxynucleotide kinases and of deoxynucleosidediphosphate kinases. 3. 3. The separate identities of deoxycytidylate and thymidylate kinases are inferred from experiments in which these enzymes were selectively inactivated. 4. 4. Both of the purine deoxynucleotide kinases also catalyze the phosphorylation of the corresponding ribonucleotides. Other properties of these enzymes have been studied.

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DO - 10.1016/0005-2787(65)90113-9

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VL - 108

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EP - 124

JO - BBA Section Nucleic Acids And Protein Synthesis

JF - BBA Section Nucleic Acids And Protein Synthesis

IS - 1

ER -

The partial purification of deoxynucleoside monophosphate kinases from L cells

Abstract

Bibliographical note

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