Virus-like particles in the ovary of an ichneumonid wasp: Purification and preliminary characterization

Peter J. Krell; Donald B. Stoltz

doi:10.1016/0042-6822(80)90454-7

Virus-like particles in the ovary of an ichneumonid wasp: Purification and preliminary characterization

Peter J. Krell, Donald B. Stoltz

Medicine

Medicine

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56 Citations (Scopus)

Abstract

Virus-like particles have been isolated from the oviducts of a parasitoid wasp, Hyposoter exiguae (Hymenoptera: Ichneumonidae). Particles are readily purified by centrifugation on either Ficoll or sucrose gradients. Double-stranded circular DNA isolated from purified particles is heterodisperse in terms of molecular weight; none of the molecules are sufficiently large to code for the aggregate of structural proteins comprising the particles. Preliminary Southern blot hybridization data suggest that there is minimal sequence homology between the different size classes of DNA examined.

Original language	English
Pages (from-to)	408-418
Number of pages	11
Journal	Virology
Volume	101
Issue number	2
DOIs	https://doi.org/10.1016/0042-6822(80)90454-7
Publication status	Published - Mar 1980

Bibliographical note

Funding Information:
We thank Dr. E. R. Oatman, Department of Entomology, University of California at Riverside, for supplying starter material for our H. eziguae colony. The technical assistance of Joan Proctor is gratefully acknowledged. This research was supported by the Medical Research Council of Canada (Grant MA-5975).

ASJC Scopus Subject Areas

Virology

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10.1016/0042-6822(80)90454-7

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abstract = "Virus-like particles have been isolated from the oviducts of a parasitoid wasp, Hyposoter exiguae (Hymenoptera: Ichneumonidae). Particles are readily purified by centrifugation on either Ficoll or sucrose gradients. Double-stranded circular DNA isolated from purified particles is heterodisperse in terms of molecular weight; none of the molecules are sufficiently large to code for the aggregate of structural proteins comprising the particles. Preliminary Southern blot hybridization data suggest that there is minimal sequence homology between the different size classes of DNA examined.",

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note = "Funding Information: We thank Dr. E. R. Oatman, Department of Entomology, University of California at Riverside, for supplying starter material for our H. eziguae colony. The technical assistance of Joan Proctor is gratefully acknowledged. This research was supported by the Medical Research Council of Canada (Grant MA-5975).",

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AU - Krell, Peter J.

AU - Stoltz, Donald B.

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AB - Virus-like particles have been isolated from the oviducts of a parasitoid wasp, Hyposoter exiguae (Hymenoptera: Ichneumonidae). Particles are readily purified by centrifugation on either Ficoll or sucrose gradients. Double-stranded circular DNA isolated from purified particles is heterodisperse in terms of molecular weight; none of the molecules are sufficiently large to code for the aggregate of structural proteins comprising the particles. Preliminary Southern blot hybridization data suggest that there is minimal sequence homology between the different size classes of DNA examined.

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Virus-like particles in the ovary of an ichneumonid wasp: Purification and preliminary characterization

Abstract

Bibliographical note

ASJC Scopus Subject Areas

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