Virus-like particles in the ovary of an ichneumonid wasp: Purification and preliminary characterization

Peter J. Krell; Donald B. Stoltz

doi:10.1016/0042-6822(80)90454-7

Virus-like particles in the ovary of an ichneumonid wasp: Purification and preliminary characterization

Peter J. Krell, Donald B. Stoltz

Medicine

Medicine

Résultat de recherche: Article › examen par les pairs

56 Citations (Scopus)

Résumé

Virus-like particles have been isolated from the oviducts of a parasitoid wasp, Hyposoter exiguae (Hymenoptera: Ichneumonidae). Particles are readily purified by centrifugation on either Ficoll or sucrose gradients. Double-stranded circular DNA isolated from purified particles is heterodisperse in terms of molecular weight; none of the molecules are sufficiently large to code for the aggregate of structural proteins comprising the particles. Preliminary Southern blot hybridization data suggest that there is minimal sequence homology between the different size classes of DNA examined.

Langue d'origine	English
Pages (de-à)	408-418
Nombre de pages	11
Journal	Virology
Volume	101
Numéro de publication	2
DOI	https://doi.org/10.1016/0042-6822(80)90454-7
Statut de publication	Published - mars 1980

Note bibliographique

Funding Information:
We thank Dr. E. R. Oatman, Department of Entomology, University of California at Riverside, for supplying starter material for our H. eziguae colony. The technical assistance of Joan Proctor is gratefully acknowledged. This research was supported by the Medical Research Council of Canada (Grant MA-5975).

ASJC Scopus Subject Areas

Virology

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10.1016/0042-6822(80)90454-7

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abstract = "Virus-like particles have been isolated from the oviducts of a parasitoid wasp, Hyposoter exiguae (Hymenoptera: Ichneumonidae). Particles are readily purified by centrifugation on either Ficoll or sucrose gradients. Double-stranded circular DNA isolated from purified particles is heterodisperse in terms of molecular weight; none of the molecules are sufficiently large to code for the aggregate of structural proteins comprising the particles. Preliminary Southern blot hybridization data suggest that there is minimal sequence homology between the different size classes of DNA examined.",

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note = "Funding Information: We thank Dr. E. R. Oatman, Department of Entomology, University of California at Riverside, for supplying starter material for our H. eziguae colony. The technical assistance of Joan Proctor is gratefully acknowledged. This research was supported by the Medical Research Council of Canada (Grant MA-5975).",

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AU - Stoltz, Donald B.

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AB - Virus-like particles have been isolated from the oviducts of a parasitoid wasp, Hyposoter exiguae (Hymenoptera: Ichneumonidae). Particles are readily purified by centrifugation on either Ficoll or sucrose gradients. Double-stranded circular DNA isolated from purified particles is heterodisperse in terms of molecular weight; none of the molecules are sufficiently large to code for the aggregate of structural proteins comprising the particles. Preliminary Southern blot hybridization data suggest that there is minimal sequence homology between the different size classes of DNA examined.

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Virus-like particles in the ovary of an ichneumonid wasp: Purification and preliminary characterization

Résumé

Note bibliographique

ASJC Scopus Subject Areas

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