A single structural type in the regular surface layer of Aeromonas salmonicida

Rafael A. Garduño, William W. Kay

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

2 Citas (Scopus)

Resumen

The surface protein array of Aeromonas salmonicida (or A-layer) appears, in negatively stained preparations, as two distinct patterns, type I and type II. Type I patterns were restricted to, and predominated in, darkly stained areas, whereas lighter staining regions exclusively displayed type II patterns. The type I morphology was faithfully reproduced in computer-simulated superimpositions of type II patterns, as was the intermediate transition zone frequently seen between the two patterns. Variations in the lattice constant of both patterns, presumably due to artifactual flattening, demonstrated that these patterns could not be distinguished on this basis. The conceptual model presented points to the type II pattern as the only single A-layer structural type. We propose the use of the terms type 1/type II to exclusively describe the morphological patterns that appear upon negative staining and the open/closed nomenclature to describe the conformations that a single structural type can adopt.

Idioma originalEnglish
Páginas (desde-hasta)202-208
Número de páginas7
PublicaciónJournal of Structural Biology
Volumen108
N.º3
DOI
EstadoPublished - 1992
Publicado de forma externa

Nota bibliográfica

Funding Information:
We are grateful to T. J. Trust, W. Baumeister, B. M. Phipps, and J. Thornton for helpful advice. These studies were supported by a GREAT Award fellowship to R.A.G. from the Science Council of British Columbia and by grants to W.W.K. from the Natural Sciences and Engineering Research Council and the Medical Research Council through the Center for Bacterial Disease Research and the British Columbia Health Care Research Foundation.

ASJC Scopus Subject Areas

  • Structural Biology

Huella

Profundice en los temas de investigación de 'A single structural type in the regular surface layer of Aeromonas salmonicida'. En conjunto forman una huella única.

Citar esto

Garduño, R. A., & Kay, W. W. (1992). A single structural type in the regular surface layer of Aeromonas salmonicida. Journal of Structural Biology, 108(3), 202-208. https://doi.org/10.1016/1047-8477(92)90020-B