Résumé
The surface protein array of Aeromonas salmonicida (or A-layer) appears, in negatively stained preparations, as two distinct patterns, type I and type II. Type I patterns were restricted to, and predominated in, darkly stained areas, whereas lighter staining regions exclusively displayed type II patterns. The type I morphology was faithfully reproduced in computer-simulated superimpositions of type II patterns, as was the intermediate transition zone frequently seen between the two patterns. Variations in the lattice constant of both patterns, presumably due to artifactual flattening, demonstrated that these patterns could not be distinguished on this basis. The conceptual model presented points to the type II pattern as the only single A-layer structural type. We propose the use of the terms type 1/type II to exclusively describe the morphological patterns that appear upon negative staining and the open/closed nomenclature to describe the conformations that a single structural type can adopt.
Langue d'origine | English |
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Pages (de-à) | 202-208 |
Nombre de pages | 7 |
Journal | Journal of Structural Biology |
Volume | 108 |
Numéro de publication | 3 |
DOI | |
Statut de publication | Published - 1992 |
Publié à l'externe | Oui |
Note bibliographique
Funding Information:We are grateful to T. J. Trust, W. Baumeister, B. M. Phipps, and J. Thornton for helpful advice. These studies were supported by a GREAT Award fellowship to R.A.G. from the Science Council of British Columbia and by grants to W.W.K. from the Natural Sciences and Engineering Research Council and the Medical Research Council through the Center for Bacterial Disease Research and the British Columbia Health Care Research Foundation.
ASJC Scopus Subject Areas
- Structural Biology