Acyl chain dependence of diacylglycerol activation of protein kinase C activity in vitro

Paola A. Marignani; Richard M. Epand; Rolf J. Sebaldt

doi:10.1006/bbrc.1996.1196

Acyl chain dependence of diacylglycerol activation of protein kinase C activity in vitro

Paola A. Marignani, Richard M. Epand, Rolf J. Sebaldt

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

65 Citas (Scopus)

Resumen

Stimulation of protein kinase C (PKC) activity in lipid vesicles in vitro was achieved by pure molecular species of diacylglycerol (DAG), specifically 1-stearoyl-2-acyl-sn-glycerol substituted with 2-arachidonoyl,2-eicosapentaenoyl or 2-docosahexaenoyl (SAG, SEG, and SDG, respectively). PKC activity was measured in lipid vesicles containing 30 mol% 1-palmitoyl-2-oleoyl-sn-glycerol-3-phospho-L-serine (POPS), 68-70 mol% 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (POPC), and 0-2 mol% DAG in the presence of 20 μM calcium. Our results demonstrate that amplification of PKC activity differs significantly among these molecular species of DAG. In particular, SDG at 0.5 mol% is more potent in increasing PKC activity than is dioleoylglycerol (DOG), SEG, or SAG, and SAG and SDG at 1.0 and 2.0 mol% have similar potencies which are greater than those of DOG or SEG. These findings demonstrate that sn-2 substitutions in DAG by specific n-3 and n-6 polyunsaturated fatty acids increase the potency of DAG to stimulate PKC activity in vitro.

Idioma original	English
Páginas (desde-hasta)	469-473
Número de páginas	5
Publicación	Biochemical and Biophysical Research Communications
Volumen	225
N.º	2
DOI	https://doi.org/10.1006/bbrc.1996.1196
Estado	Published - ago. 14 1996
Publicado de forma externa	Sí

Nota bibliográfica

Funding Information:
This work was supported by grants from the Arthritis Society of Canada. R.J.S. is the recipient of a Career Award from the Pharmaceutical Manufacturers’ Association of Canada-Health Research Foundation and the Medical Research Council of Canada. P.A.M. is the recipient of a Graduate Research Studentship from the Pharmaceutical Manufacturers’ Association of Canada-Health Research Foundation and the Medical Research Council of Canada. We are greatful to Dr. Klaus Gawrisch for providing us with his NMR results (25) prior to publication.

ASJC Scopus Subject Areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Acceder al documento

10.1006/bbrc.1996.1196

Otros archivos y enlaces

Citar esto

@article{fc639ce1a7eb44cc9e607de2746839c0,

title = "Acyl chain dependence of diacylglycerol activation of protein kinase C activity in vitro",

abstract = "Stimulation of protein kinase C (PKC) activity in lipid vesicles in vitro was achieved by pure molecular species of diacylglycerol (DAG), specifically 1-stearoyl-2-acyl-sn-glycerol substituted with 2-arachidonoyl,2-eicosapentaenoyl or 2-docosahexaenoyl (SAG, SEG, and SDG, respectively). PKC activity was measured in lipid vesicles containing 30 mol% 1-palmitoyl-2-oleoyl-sn-glycerol-3-phospho-L-serine (POPS), 68-70 mol% 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (POPC), and 0-2 mol% DAG in the presence of 20 μM calcium. Our results demonstrate that amplification of PKC activity differs significantly among these molecular species of DAG. In particular, SDG at 0.5 mol% is more potent in increasing PKC activity than is dioleoylglycerol (DOG), SEG, or SAG, and SAG and SDG at 1.0 and 2.0 mol% have similar potencies which are greater than those of DOG or SEG. These findings demonstrate that sn-2 substitutions in DAG by specific n-3 and n-6 polyunsaturated fatty acids increase the potency of DAG to stimulate PKC activity in vitro.",

author = "Marignani, {Paola A.} and Epand, {Richard M.} and Sebaldt, {Rolf J.}",

note = "Funding Information: This work was supported by grants from the Arthritis Society of Canada. R.J.S. is the recipient of a Career Award from the Pharmaceutical Manufacturers{\textquoteright} Association of Canada-Health Research Foundation and the Medical Research Council of Canada. P.A.M. is the recipient of a Graduate Research Studentship from the Pharmaceutical Manufacturers{\textquoteright} Association of Canada-Health Research Foundation and the Medical Research Council of Canada. We are greatful to Dr. Klaus Gawrisch for providing us with his NMR results (25) prior to publication.",

year = "1996",

month = aug,

day = "14",

doi = "10.1006/bbrc.1996.1196",

language = "English",

volume = "225",

pages = "469--473",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Acyl chain dependence of diacylglycerol activation of protein kinase C activity in vitro

AU - Marignani, Paola A.

AU - Epand, Richard M.

AU - Sebaldt, Rolf J.

N1 - Funding Information: This work was supported by grants from the Arthritis Society of Canada. R.J.S. is the recipient of a Career Award from the Pharmaceutical Manufacturers’ Association of Canada-Health Research Foundation and the Medical Research Council of Canada. P.A.M. is the recipient of a Graduate Research Studentship from the Pharmaceutical Manufacturers’ Association of Canada-Health Research Foundation and the Medical Research Council of Canada. We are greatful to Dr. Klaus Gawrisch for providing us with his NMR results (25) prior to publication.

PY - 1996/8/14

Y1 - 1996/8/14

N2 - Stimulation of protein kinase C (PKC) activity in lipid vesicles in vitro was achieved by pure molecular species of diacylglycerol (DAG), specifically 1-stearoyl-2-acyl-sn-glycerol substituted with 2-arachidonoyl,2-eicosapentaenoyl or 2-docosahexaenoyl (SAG, SEG, and SDG, respectively). PKC activity was measured in lipid vesicles containing 30 mol% 1-palmitoyl-2-oleoyl-sn-glycerol-3-phospho-L-serine (POPS), 68-70 mol% 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (POPC), and 0-2 mol% DAG in the presence of 20 μM calcium. Our results demonstrate that amplification of PKC activity differs significantly among these molecular species of DAG. In particular, SDG at 0.5 mol% is more potent in increasing PKC activity than is dioleoylglycerol (DOG), SEG, or SAG, and SAG and SDG at 1.0 and 2.0 mol% have similar potencies which are greater than those of DOG or SEG. These findings demonstrate that sn-2 substitutions in DAG by specific n-3 and n-6 polyunsaturated fatty acids increase the potency of DAG to stimulate PKC activity in vitro.

AB - Stimulation of protein kinase C (PKC) activity in lipid vesicles in vitro was achieved by pure molecular species of diacylglycerol (DAG), specifically 1-stearoyl-2-acyl-sn-glycerol substituted with 2-arachidonoyl,2-eicosapentaenoyl or 2-docosahexaenoyl (SAG, SEG, and SDG, respectively). PKC activity was measured in lipid vesicles containing 30 mol% 1-palmitoyl-2-oleoyl-sn-glycerol-3-phospho-L-serine (POPS), 68-70 mol% 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (POPC), and 0-2 mol% DAG in the presence of 20 μM calcium. Our results demonstrate that amplification of PKC activity differs significantly among these molecular species of DAG. In particular, SDG at 0.5 mol% is more potent in increasing PKC activity than is dioleoylglycerol (DOG), SEG, or SAG, and SAG and SDG at 1.0 and 2.0 mol% have similar potencies which are greater than those of DOG or SEG. These findings demonstrate that sn-2 substitutions in DAG by specific n-3 and n-6 polyunsaturated fatty acids increase the potency of DAG to stimulate PKC activity in vitro.

UR - http://www.scopus.com/inward/record.url?scp=0030583240&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030583240&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1996.1196

DO - 10.1006/bbrc.1996.1196

M3 - Article

C2 - 8753785

AN - SCOPUS:0030583240

SN - 0006-291X

VL - 225

SP - 469

EP - 473

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Acyl chain dependence of diacylglycerol activation of protein kinase C activity in vitro

Resumen

Nota bibliográfica

ASJC Scopus Subject Areas

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto