Alternative splicing generates multiple mRNA forms of the acetylcholine receptor γ-subunit in rat muscle

Alfredo Villarroel

doi:10.1016/S0014-5793(99)00012-5

Alternative splicing generates multiple mRNA forms of the acetylcholine receptor γ-subunit in rat muscle

Alfredo Villarroel

Medicine

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

7 Citas (Scopus)

Resumen

The fetal type acetylcholine receptor, composed of the αβγδ subunits, has shown a highly variable channel kinetics during postnatal development. We examine the hypothesis whether such a variability could result from multiple channel forms, differing in the N-terminus of the γ-subunit. RT-PCR revealed, in addition to the full-length mRNA, three new forms lacking exon 4. One of them in addition lacks 19 nucleotides from exon 5, predicting a complete subunit, with a 43 residues shorter N-terminus. A third one lacking the complete exon 5 predicts a subunit without transmembrane segments. These forms, generated by alternative splicing, may account for the kinetic variability of the acetylcholine receptor channel. Copyright (C) 1999 Federation of European Biochemical Societies.

Idioma original	English
Páginas (desde-hasta)	381-384
Número de páginas	4
Publicación	FEBS Letters
Volumen	443
N.º	3
DOI	https://doi.org/10.1016/S0014-5793(99)00012-5
Estado	Published - ene. 29 1999

Nota bibliográfica

Funding Information:
I am grateful to Ms. J. Froom for expert technical assistance, and to Dr. P. Murphy for critical reading of the manuscript. This work was supported by the Medical Research Council of Canada, Grant MT-14457.

ASJC Scopus Subject Areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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10.1016/S0014-5793(99)00012-5

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Villarroel, A. (1999). Alternative splicing generates multiple mRNA forms of the acetylcholine receptor γ-subunit in rat muscle. FEBS Letters, 443(3), 381-384. https://doi.org/10.1016/S0014-5793(99)00012-5

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abstract = "The fetal type acetylcholine receptor, composed of the αβγδ subunits, has shown a highly variable channel kinetics during postnatal development. We examine the hypothesis whether such a variability could result from multiple channel forms, differing in the N-terminus of the γ-subunit. RT-PCR revealed, in addition to the full-length mRNA, three new forms lacking exon 4. One of them in addition lacks 19 nucleotides from exon 5, predicting a complete subunit, with a 43 residues shorter N-terminus. A third one lacking the complete exon 5 predicts a subunit without transmembrane segments. These forms, generated by alternative splicing, may account for the kinetic variability of the acetylcholine receptor channel. Copyright (C) 1999 Federation of European Biochemical Societies.",

author = "Alfredo Villarroel",

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N2 - The fetal type acetylcholine receptor, composed of the αβγδ subunits, has shown a highly variable channel kinetics during postnatal development. We examine the hypothesis whether such a variability could result from multiple channel forms, differing in the N-terminus of the γ-subunit. RT-PCR revealed, in addition to the full-length mRNA, three new forms lacking exon 4. One of them in addition lacks 19 nucleotides from exon 5, predicting a complete subunit, with a 43 residues shorter N-terminus. A third one lacking the complete exon 5 predicts a subunit without transmembrane segments. These forms, generated by alternative splicing, may account for the kinetic variability of the acetylcholine receptor channel. Copyright (C) 1999 Federation of European Biochemical Societies.

AB - The fetal type acetylcholine receptor, composed of the αβγδ subunits, has shown a highly variable channel kinetics during postnatal development. We examine the hypothesis whether such a variability could result from multiple channel forms, differing in the N-terminus of the γ-subunit. RT-PCR revealed, in addition to the full-length mRNA, three new forms lacking exon 4. One of them in addition lacks 19 nucleotides from exon 5, predicting a complete subunit, with a 43 residues shorter N-terminus. A third one lacking the complete exon 5 predicts a subunit without transmembrane segments. These forms, generated by alternative splicing, may account for the kinetic variability of the acetylcholine receptor channel. Copyright (C) 1999 Federation of European Biochemical Societies.

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Alternative splicing generates multiple mRNA forms of the acetylcholine receptor γ-subunit in rat muscle

Resumen

Nota bibliográfica

ASJC Scopus Subject Areas

PubMed: MeSH publication types

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