Résumé
The fetal type acetylcholine receptor, composed of the αβγδ subunits, has shown a highly variable channel kinetics during postnatal development. We examine the hypothesis whether such a variability could result from multiple channel forms, differing in the N-terminus of the γ-subunit. RT-PCR revealed, in addition to the full-length mRNA, three new forms lacking exon 4. One of them in addition lacks 19 nucleotides from exon 5, predicting a complete subunit, with a 43 residues shorter N-terminus. A third one lacking the complete exon 5 predicts a subunit without transmembrane segments. These forms, generated by alternative splicing, may account for the kinetic variability of the acetylcholine receptor channel. Copyright (C) 1999 Federation of European Biochemical Societies.
| Langue d'origine | English |
|---|---|
| Pages (de-à) | 381-384 |
| Nombre de pages | 4 |
| Journal | FEBS Letters |
| Volume | 443 |
| Numéro de publication | 3 |
| DOI | |
| Statut de publication | Published - janv. 29 1999 |
Note bibliographique
Funding Information:I am grateful to Ms. J. Froom for expert technical assistance, and to Dr. P. Murphy for critical reading of the manuscript. This work was supported by the Medical Research Council of Canada, Grant MT-14457.
ASJC Scopus Subject Areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology
PubMed: MeSH publication types
- Journal Article
- Research Support, Non-U.S. Gov't