Development and characterization of 15 novel microsatellite markers for the freshwater fish Galaxias platei

Ivan Vera-Escalona; Ian G. Paterson; Evelyn Habit; Daniel E. Ruzzante

doi:10.1007/s12686-014-0236-2

Development and characterization of 15 novel microsatellite markers for the freshwater fish Galaxias platei

Ivan Vera-Escalona, Ian G. Paterson, Evelyn Habit, Daniel E. Ruzzante

Medicine

Medicine

Producción científica: Contribución a una revista › Carta › revisión exhaustiva

2 Citas (Scopus)

Resumen

Galaxias platei is a fish species restricted to lakes and rivers from Patagonia. In this study, 15 novel microsatellite loci were developed and characterized for 64 individuals of G. platei collected from a Patagonian lake. Eight loci were polymorphic and seven were monomorphic. The number of alleles in the polymorphic loci ranged from 3 to 6, and the expected and observed heterozygosities ranged from 0.08 to 0.68, and 0.08 to 0.62, respectively. These new markers are being used in an ongoing landscape genetics study on G. platei.

Idioma original	English
Publicación	Conservation Genetics Resources
Volumen	6
N.º	4
DOI	https://doi.org/10.1007/s12686-014-0236-2
Estado	Published - dic. 2014

Nota bibliográfica

Funding Information:
Acknowledgments We thank Jorge González for assistance collecting samples. Funding for this research was provided by an NSERC to DER. DNA sequence data were generated on an Illumina MiSeq sequencer acquired with the support of a generous bequest from Elizabeth A. Nielsen to the Marine Gene Probe Laboratory.

Publisher Copyright:
© 2014, Springer Science+Business Media Dordrecht.

ASJC Scopus Subject Areas

Ecology, Evolution, Behavior and Systematics
Genetics

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abstract = "Galaxias platei is a fish species restricted to lakes and rivers from Patagonia. In this study, 15 novel microsatellite loci were developed and characterized for 64 individuals of G. platei collected from a Patagonian lake. Eight loci were polymorphic and seven were monomorphic. The number of alleles in the polymorphic loci ranged from 3 to 6, and the expected and observed heterozygosities ranged from 0.08 to 0.68, and 0.08 to 0.62, respectively. These new markers are being used in an ongoing landscape genetics study on G. platei.",

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AB - Galaxias platei is a fish species restricted to lakes and rivers from Patagonia. In this study, 15 novel microsatellite loci were developed and characterized for 64 individuals of G. platei collected from a Patagonian lake. Eight loci were polymorphic and seven were monomorphic. The number of alleles in the polymorphic loci ranged from 3 to 6, and the expected and observed heterozygosities ranged from 0.08 to 0.68, and 0.08 to 0.62, respectively. These new markers are being used in an ongoing landscape genetics study on G. platei.

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Development and characterization of 15 novel microsatellite markers for the freshwater fish Galaxias platei

Resumen

Nota bibliográfica

ASJC Scopus Subject Areas

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