Development and characterization of 15 novel microsatellite markers for the freshwater fish Galaxias platei

Ivan Vera-Escalona; Ian G. Paterson; Evelyn Habit; Daniel E. Ruzzante

doi:10.1007/s12686-014-0236-2

Development and characterization of 15 novel microsatellite markers for the freshwater fish Galaxias platei

Ivan Vera-Escalona, Ian G. Paterson, Evelyn Habit, Daniel E. Ruzzante

Medicine

Medicine

Résultat de recherche: Letter › examen par les pairs

2 Citations (Scopus)

Résumé

Galaxias platei is a fish species restricted to lakes and rivers from Patagonia. In this study, 15 novel microsatellite loci were developed and characterized for 64 individuals of G. platei collected from a Patagonian lake. Eight loci were polymorphic and seven were monomorphic. The number of alleles in the polymorphic loci ranged from 3 to 6, and the expected and observed heterozygosities ranged from 0.08 to 0.68, and 0.08 to 0.62, respectively. These new markers are being used in an ongoing landscape genetics study on G. platei.

Langue d'origine	English
Journal	Conservation Genetics Resources
Volume	6
Numéro de publication	4
DOI	https://doi.org/10.1007/s12686-014-0236-2
Statut de publication	Published - déc. 2014

Note bibliographique

Funding Information:
Acknowledgments We thank Jorge González for assistance collecting samples. Funding for this research was provided by an NSERC to DER. DNA sequence data were generated on an Illumina MiSeq sequencer acquired with the support of a generous bequest from Elizabeth A. Nielsen to the Marine Gene Probe Laboratory.

Publisher Copyright:
© 2014, Springer Science+Business Media Dordrecht.

ASJC Scopus Subject Areas

Ecology, Evolution, Behavior and Systematics
Genetics

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10.1007/s12686-014-0236-2

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abstract = "Galaxias platei is a fish species restricted to lakes and rivers from Patagonia. In this study, 15 novel microsatellite loci were developed and characterized for 64 individuals of G. platei collected from a Patagonian lake. Eight loci were polymorphic and seven were monomorphic. The number of alleles in the polymorphic loci ranged from 3 to 6, and the expected and observed heterozygosities ranged from 0.08 to 0.68, and 0.08 to 0.62, respectively. These new markers are being used in an ongoing landscape genetics study on G. platei.",

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AU - Vera-Escalona, Ivan

AU - Paterson, Ian G.

AU - Habit, Evelyn

AU - Ruzzante, Daniel E.

N1 - Funding Information: Acknowledgments We thank Jorge González for assistance collecting samples. Funding for this research was provided by an NSERC to DER. DNA sequence data were generated on an Illumina MiSeq sequencer acquired with the support of a generous bequest from Elizabeth A. Nielsen to the Marine Gene Probe Laboratory. Publisher Copyright: © 2014, Springer Science+Business Media Dordrecht.

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N2 - Galaxias platei is a fish species restricted to lakes and rivers from Patagonia. In this study, 15 novel microsatellite loci were developed and characterized for 64 individuals of G. platei collected from a Patagonian lake. Eight loci were polymorphic and seven were monomorphic. The number of alleles in the polymorphic loci ranged from 3 to 6, and the expected and observed heterozygosities ranged from 0.08 to 0.68, and 0.08 to 0.62, respectively. These new markers are being used in an ongoing landscape genetics study on G. platei.

AB - Galaxias platei is a fish species restricted to lakes and rivers from Patagonia. In this study, 15 novel microsatellite loci were developed and characterized for 64 individuals of G. platei collected from a Patagonian lake. Eight loci were polymorphic and seven were monomorphic. The number of alleles in the polymorphic loci ranged from 3 to 6, and the expected and observed heterozygosities ranged from 0.08 to 0.68, and 0.08 to 0.62, respectively. These new markers are being used in an ongoing landscape genetics study on G. platei.

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Development and characterization of 15 novel microsatellite markers for the freshwater fish Galaxias platei

Résumé

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ASJC Scopus Subject Areas

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