Electron microscopy of legionella and legionella-infected cells

Gary Faulkner, Rafael A. Garduño

Producción científica: Capítulo en Libro/Reporte/Acta de conferenciaCapítulo

5 Citas (Scopus)

Resumen

Those investigators who study the morphology of Legionella and Legionella-infected cells have greatly benefited from the superior resolution afforded by electron microscopy (EM). It can also be said with confidence that EM will continue to reveal as yet to be discovered features of this fascinating intracellular pathogen. In this chapter we detail our practical experience in the application of three transmission electron microscopy (TEM) techniques to the study of Legionella: conventional ultrastructural analysis, immuno-gold labeling, and negative staining. Each of these techniques has particular, well-defined applications, which are discussed in the context of our in-house developed methods. We invite researchers to try the methods given here in the study of Legionella, and adopt TEM as part of their research tools arsenal.

Idioma originalEnglish
Título de la publicación alojadaLegionella
Subtítulo de la publicación alojadaMethods and Protocols
EditorialHumana Press Inc.
Páginas279-307
Número de páginas29
ISBN (versión impresa)9781627031608
DOI
EstadoPublished - 2013
Publicado de forma externa

Serie de la publicación

NombreMethods in Molecular Biology
Volumen954
ISSN (versión impresa)1064-3745

ASJC Scopus Subject Areas

  • Molecular Biology
  • Genetics

PubMed: MeSH publication types

  • Journal Article

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