Electron microscopy of legionella and legionella-infected cells

Gary Faulkner, Rafael A. Garduño

Résultat de recherche: Chapter

5 Citations (Scopus)

Résumé

Those investigators who study the morphology of Legionella and Legionella-infected cells have greatly benefited from the superior resolution afforded by electron microscopy (EM). It can also be said with confidence that EM will continue to reveal as yet to be discovered features of this fascinating intracellular pathogen. In this chapter we detail our practical experience in the application of three transmission electron microscopy (TEM) techniques to the study of Legionella: conventional ultrastructural analysis, immuno-gold labeling, and negative staining. Each of these techniques has particular, well-defined applications, which are discussed in the context of our in-house developed methods. We invite researchers to try the methods given here in the study of Legionella, and adopt TEM as part of their research tools arsenal.

Langue d'origineEnglish
Titre de la publication principaleLegionella
Sous-titre de la publication principaleMethods and Protocols
Maison d'éditionHumana Press Inc.
Pages279-307
Nombre de pages29
ISBN (imprimé)9781627031608
DOI
Statut de publicationPublished - 2013
Publié à l'externeOui

Séries de publication

PrénomMethods in Molecular Biology
Volume954
ISSN (imprimé)1064-3745

ASJC Scopus Subject Areas

  • Molecular Biology
  • Genetics

PubMed: MeSH publication types

  • Journal Article

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