Implications of heterogeneous bead behavior on cell mechanical properties measured with magnetic twisting cytometry

Ben Fabry; Geoffrey N. Maksym; Rolf D. Hubmayr; James P. Butler; Jeffrey J. Fredberg

doi:10.1016/S0304-8853(98)00564-2

Implications of heterogeneous bead behavior on cell mechanical properties measured with magnetic twisting cytometry

Ben Fabry, Geoffrey N. Maksym, Rolf D. Hubmayr, James P. Butler, Jeffrey J. Fredberg

Producción científica: Contribución a una revista › Artículo de la conferencia › revisión exhaustiva

83 Citas (Scopus)

Resumen

Magnetic twisting cytometry (MTC) measures cellular mechanical properties, such as cell stiffness and viscosity, by applying mechanical stress to specific cell surface receptors via ligand-coated ferromagnetic beads. MTC measures simultaneously the rotation of approximately 50,000 beads attached to 20,000-40,000 cells. Here we show direct evidence of heterogeneous bead behavior and examine its consequences in the interpretation of cell mechanical properties.

Idioma original	English
Páginas (desde-hasta)	120-125
Número de páginas	6
Publicación	Journal of Magnetism and Magnetic Materials
Volumen	194
N.º	1
DOI	https://doi.org/10.1016/S0304-8853(98)00564-2
Estado	Published - abr. 1999
Publicado de forma externa	Sí
Evento	Proceedings of 1998 2nd International Conference on Scientific and Clinical Applications of Magnetic Carriers - Cleveland, OH, USA Duración: may. 28 1998 → may. 30 1998

Nota bibliográfica

Funding Information:
We thank Tori Hatch for her invaluable help with scanning EM. Ning Wang has supplied the BCE cells and materials for MTC and helped us mastering the MTC technique. Rey Panettieri (University of Pennsylvania) has supplied the HASM cells. We thank In Lim, Johanne Laporte, Paul Moore and Jianxin Chen for technical assistance with the cell preparation. This work was supported by the Swiss National Foundation, Deutsche Forschungsgemeinschaft, NIH and NSERC Canada.

ASJC Scopus Subject Areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics

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10.1016/S0304-8853(98)00564-2

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Implications of heterogeneous bead behavior on cell mechanical properties measured with magnetic twisting cytometry. / Fabry, Ben; Maksym, Geoffrey N.; Hubmayr, Rolf D. et al.
En: Journal of Magnetism and Magnetic Materials, Vol. 194, N.º 1, 04.1999, p. 120-125.

Producción científica: Contribución a una revista › Artículo de la conferencia › revisión exhaustiva

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abstract = "Magnetic twisting cytometry (MTC) measures cellular mechanical properties, such as cell stiffness and viscosity, by applying mechanical stress to specific cell surface receptors via ligand-coated ferromagnetic beads. MTC measures simultaneously the rotation of approximately 50,000 beads attached to 20,000-40,000 cells. Here we show direct evidence of heterogeneous bead behavior and examine its consequences in the interpretation of cell mechanical properties.",

author = "Ben Fabry and Maksym, {Geoffrey N.} and Hubmayr, {Rolf D.} and Butler, {James P.} and Fredberg, {Jeffrey J.}",

note = "Funding Information: We thank Tori Hatch for her invaluable help with scanning EM. Ning Wang has supplied the BCE cells and materials for MTC and helped us mastering the MTC technique. Rey Panettieri (University of Pennsylvania) has supplied the HASM cells. We thank In Lim, Johanne Laporte, Paul Moore and Jianxin Chen for technical assistance with the cell preparation. This work was supported by the Swiss National Foundation, Deutsche Forschungsgemeinschaft, NIH and NSERC Canada.; Proceedings of 1998 2nd International Conference on Scientific and Clinical Applications of Magnetic Carriers ; Conference date: 28-05-1998 Through 30-05-1998",

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AU - Fredberg, Jeffrey J.

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AB - Magnetic twisting cytometry (MTC) measures cellular mechanical properties, such as cell stiffness and viscosity, by applying mechanical stress to specific cell surface receptors via ligand-coated ferromagnetic beads. MTC measures simultaneously the rotation of approximately 50,000 beads attached to 20,000-40,000 cells. Here we show direct evidence of heterogeneous bead behavior and examine its consequences in the interpretation of cell mechanical properties.

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Implications of heterogeneous bead behavior on cell mechanical properties measured with magnetic twisting cytometry

Resumen

Nota bibliográfica

ASJC Scopus Subject Areas

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