The metabolism of very low density lipoprotein and chylomicrons by purified lipoprotein lipase from rat heart and adipose tissue

Peter J. Dolphin; David Rubinstein

doi:10.1016/0006-291X(74)90618-4

The metabolism of very low density lipoprotein and chylomicrons by purified lipoprotein lipase from rat heart and adipose tissue

Peter J. Dolphin, David Rubinstein

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

14 Citas (Scopus)

Resumen

Crude lipoprotein lipase, extracted from rat adipose tissue or heart acetone-ether powders, was purified about 300 and 350 fold respectively by affinity chromatography. Artifactual increments in the density of very low density lipoprotein, noted after incubation with the crude lipoprotein lipase extract from adipose tissue, were abolished when the purified enzyme was used. Purified enzymes from both tissues showed similar modifications of activity in the presence of activators and inhibitors. The triglyceride moieties of various natural substrates were preferentially hydrolysed in the order Very low density lipoprotein > Serum chylomicrons > Thoracic duct chylomicrons by both enzymes.

Idioma original	English
Páginas (desde-hasta)	808-814
Número de páginas	7
Publicación	Biochemical and Biophysical Research Communications
Volumen	57
N.º	3
DOI	https://doi.org/10.1016/0006-291X(74)90618-4
Estado	Published - abr. 8 1974
Publicado de forma externa	Sí

Nota bibliográfica

Funding Information:
ACKNOWLEDGEMENTS: The authors thank assistance. Dr. P.J. Dolphin is a Canadian supported by grants from the Medical Research Foundation.

ASJC Scopus Subject Areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "Crude lipoprotein lipase, extracted from rat adipose tissue or heart acetone-ether powders, was purified about 300 and 350 fold respectively by affinity chromatography. Artifactual increments in the density of very low density lipoprotein, noted after incubation with the crude lipoprotein lipase extract from adipose tissue, were abolished when the purified enzyme was used. Purified enzymes from both tissues showed similar modifications of activity in the presence of activators and inhibitors. The triglyceride moieties of various natural substrates were preferentially hydrolysed in the order Very low density lipoprotein > Serum chylomicrons > Thoracic duct chylomicrons by both enzymes.",

author = "Dolphin, {Peter J.} and David Rubinstein",

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AU - Rubinstein, David

N1 - Funding Information: ACKNOWLEDGEMENTS: The authors thank assistance. Dr. P.J. Dolphin is a Canadian supported by grants from the Medical Research Foundation.

PY - 1974/4/8

Y1 - 1974/4/8

N2 - Crude lipoprotein lipase, extracted from rat adipose tissue or heart acetone-ether powders, was purified about 300 and 350 fold respectively by affinity chromatography. Artifactual increments in the density of very low density lipoprotein, noted after incubation with the crude lipoprotein lipase extract from adipose tissue, were abolished when the purified enzyme was used. Purified enzymes from both tissues showed similar modifications of activity in the presence of activators and inhibitors. The triglyceride moieties of various natural substrates were preferentially hydrolysed in the order Very low density lipoprotein > Serum chylomicrons > Thoracic duct chylomicrons by both enzymes.

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The metabolism of very low density lipoprotein and chylomicrons by purified lipoprotein lipase from rat heart and adipose tissue

Resumen

Nota bibliográfica

ASJC Scopus Subject Areas

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