The metabolism of very low density lipoprotein and chylomicrons by purified lipoprotein lipase from rat heart and adipose tissue

Peter J. Dolphin; David Rubinstein

doi:10.1016/0006-291X(74)90618-4

The metabolism of very low density lipoprotein and chylomicrons by purified lipoprotein lipase from rat heart and adipose tissue

Peter J. Dolphin, David Rubinstein

Résultat de recherche: Article › examen par les pairs

14 Citations (Scopus)

Résumé

Crude lipoprotein lipase, extracted from rat adipose tissue or heart acetone-ether powders, was purified about 300 and 350 fold respectively by affinity chromatography. Artifactual increments in the density of very low density lipoprotein, noted after incubation with the crude lipoprotein lipase extract from adipose tissue, were abolished when the purified enzyme was used. Purified enzymes from both tissues showed similar modifications of activity in the presence of activators and inhibitors. The triglyceride moieties of various natural substrates were preferentially hydrolysed in the order Very low density lipoprotein > Serum chylomicrons > Thoracic duct chylomicrons by both enzymes.

Langue d'origine	English
Pages (de-à)	808-814
Nombre de pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	57
Numéro de publication	3
DOI	https://doi.org/10.1016/0006-291X(74)90618-4
Statut de publication	Published - avr. 8 1974
Publié à l'externe	Oui

Note bibliographique

Funding Information:
ACKNOWLEDGEMENTS: The authors thank assistance. Dr. P.J. Dolphin is a Canadian supported by grants from the Medical Research Foundation.

ASJC Scopus Subject Areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(74)90618-4

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abstract = "Crude lipoprotein lipase, extracted from rat adipose tissue or heart acetone-ether powders, was purified about 300 and 350 fold respectively by affinity chromatography. Artifactual increments in the density of very low density lipoprotein, noted after incubation with the crude lipoprotein lipase extract from adipose tissue, were abolished when the purified enzyme was used. Purified enzymes from both tissues showed similar modifications of activity in the presence of activators and inhibitors. The triglyceride moieties of various natural substrates were preferentially hydrolysed in the order Very low density lipoprotein > Serum chylomicrons > Thoracic duct chylomicrons by both enzymes.",

author = "Dolphin, {Peter J.} and David Rubinstein",

note = "Funding Information: ACKNOWLEDGEMENTS: The authors thank assistance. Dr. P.J. Dolphin is a Canadian supported by grants from the Medical Research Foundation.",

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T1 - The metabolism of very low density lipoprotein and chylomicrons by purified lipoprotein lipase from rat heart and adipose tissue

AU - Dolphin, Peter J.

AU - Rubinstein, David

N1 - Funding Information: ACKNOWLEDGEMENTS: The authors thank assistance. Dr. P.J. Dolphin is a Canadian supported by grants from the Medical Research Foundation.

PY - 1974/4/8

Y1 - 1974/4/8

N2 - Crude lipoprotein lipase, extracted from rat adipose tissue or heart acetone-ether powders, was purified about 300 and 350 fold respectively by affinity chromatography. Artifactual increments in the density of very low density lipoprotein, noted after incubation with the crude lipoprotein lipase extract from adipose tissue, were abolished when the purified enzyme was used. Purified enzymes from both tissues showed similar modifications of activity in the presence of activators and inhibitors. The triglyceride moieties of various natural substrates were preferentially hydrolysed in the order Very low density lipoprotein > Serum chylomicrons > Thoracic duct chylomicrons by both enzymes.

AB - Crude lipoprotein lipase, extracted from rat adipose tissue or heart acetone-ether powders, was purified about 300 and 350 fold respectively by affinity chromatography. Artifactual increments in the density of very low density lipoprotein, noted after incubation with the crude lipoprotein lipase extract from adipose tissue, were abolished when the purified enzyme was used. Purified enzymes from both tissues showed similar modifications of activity in the presence of activators and inhibitors. The triglyceride moieties of various natural substrates were preferentially hydrolysed in the order Very low density lipoprotein > Serum chylomicrons > Thoracic duct chylomicrons by both enzymes.

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JO - Biochemical and Biophysical Research Communications

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The metabolism of very low density lipoprotein and chylomicrons by purified lipoprotein lipase from rat heart and adipose tissue

Résumé

Note bibliographique

ASJC Scopus Subject Areas

Accès au document

Autres fichiers et liens

Empreinte numérique

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