THERMOMECHANICAL CHARACTERIZATION OF COLLAGEN CROSSLINKING IN DEVELOPING CARDIOVASCULAR TISSUES

Sarah M. Wells; Wendy A. Naimark; J. Michael Lee

doi:10.1115/IMECE1999-0392

THERMOMECHANICAL CHARACTERIZATION OF COLLAGEN CROSSLINKING IN DEVELOPING CARDIOVASCULAR TISSUES

Sarah M. Wells, Wendy A. Naimark, J. Michael Lee

Medicine

Medicine

Producción científica: Capítulo en Libro/Reporte/Acta de conferencia › Contribución a la conferencia

Resumen

We have used hydrothermal isometric tension (HIT) tests to assess developmental changes in collagen crosslinking in the ovine thoracic aorta and pericardium. Tissue strips were loaded under isometric tension and heated to 90°C for 3 lirs. Load relaxation at this temperature is associated with collagen hydrolysis and chain slippage, the half-time of load decay (t_1/2) being an indicator of collagen crosslinking. t_1/2was computed before and after tissues were treated with NaBH₄which stabilizes immature crosslinks. The t_1/2of untreated tissue increased from the lamb to the adult, indicating that collagen crosslinking increased postnatally. Furthermore, the t_1/2of NaBH₄-treated lamb tissue (3d pericardium and 21d aorta) was similar to that of untreated adult tissue, suggesting that much immature crosslinking in the lamb is stabilized postnatally. These observations suggest (i) increased crosslinking occurs postnatally, (ii) this increase is largely due to the conversion of immature crosslinks into their mature, heat stable form (Naimark et al., 1998, Wells et al., 1999).

Idioma original	English
Título de la publicación alojada	Advances in Bioengineering
Editorial	American Society of Mechanical Engineers (ASME)
Páginas	91-92
Número de páginas	2
ISBN (versión digital)	9780791816400
DOI	https://doi.org/10.1115/IMECE1999-0392
Estado	Published - 1999
Evento	ASME 1999 International Mechanical Engineering Congress and Exposition, IMECE 1999 - Nashville, United States Duración: nov. 14 1999 → nov. 19 1999

Serie de la publicación

Nombre	ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)
Volumen	1999-D

Conference

Conference	ASME 1999 International Mechanical Engineering Congress and Exposition, IMECE 1999
País/Territorio	United States
Ciudad	Nashville
Período	11/14/99 → 11/19/99

Nota bibliográfica

Funding Information:
Funded by the Heart and Stroke Foundation of Ontario and the Natural Sciences and Engineering Research Council of Canada.

Publisher Copyright:
© 1999 American Society of Mechanical Engineers (ASME). All rights reserved.

ASJC Scopus Subject Areas

Mechanical Engineering

Acceder al documento

10.1115/IMECE1999-0392

Otros archivos y enlaces

Citar esto

Wells, S. M., Naimark, W. A., & Lee, J. M. (1999). THERMOMECHANICAL CHARACTERIZATION OF COLLAGEN CROSSLINKING IN DEVELOPING CARDIOVASCULAR TISSUES. En Advances in Bioengineering (pp. 91-92). (ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE); Vol. 1999-D). American Society of Mechanical Engineers (ASME). https://doi.org/10.1115/IMECE1999-0392

THERMOMECHANICAL CHARACTERIZATION OF COLLAGEN CROSSLINKING IN DEVELOPING CARDIOVASCULAR TISSUES. / Wells, Sarah M.; Naimark, Wendy A.; Lee, J. Michael.
Advances in Bioengineering. American Society of Mechanical Engineers (ASME), 1999. p. 91-92 (ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE); Vol. 1999-D).

Producción científica: Capítulo en Libro/Reporte/Acta de conferencia › Contribución a la conferencia

Wells, SM, Naimark, WA & Lee, JM 1999, THERMOMECHANICAL CHARACTERIZATION OF COLLAGEN CROSSLINKING IN DEVELOPING CARDIOVASCULAR TISSUES. En Advances in Bioengineering. ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE), vol. 1999-D, American Society of Mechanical Engineers (ASME), pp. 91-92, ASME 1999 International Mechanical Engineering Congress and Exposition, IMECE 1999, Nashville, United States, 11/14/99. https://doi.org/10.1115/IMECE1999-0392

@inproceedings{3f20ac6447a44a8c84c7099a0de88b4b,

title = "THERMOMECHANICAL CHARACTERIZATION OF COLLAGEN CROSSLINKING IN DEVELOPING CARDIOVASCULAR TISSUES",

abstract = "We have used hydrothermal isometric tension (HIT) tests to assess developmental changes in collagen crosslinking in the ovine thoracic aorta and pericardium. Tissue strips were loaded under isometric tension and heated to 90°C for 3 lirs. Load relaxation at this temperature is associated with collagen hydrolysis and chain slippage, the half-time of load decay (t1/2) being an indicator of collagen crosslinking. t1/2was computed before and after tissues were treated with NaBH4which stabilizes immature crosslinks. The t1/2of untreated tissue increased from the lamb to the adult, indicating that collagen crosslinking increased postnatally. Furthermore, the t1/2of NaBH4-treated lamb tissue (3d pericardium and 21d aorta) was similar to that of untreated adult tissue, suggesting that much immature crosslinking in the lamb is stabilized postnatally. These observations suggest (i) increased crosslinking occurs postnatally, (ii) this increase is largely due to the conversion of immature crosslinks into their mature, heat stable form (Naimark et al., 1998, Wells et al., 1999).",

author = "Wells, {Sarah M.} and Naimark, {Wendy A.} and Lee, {J. Michael}",

note = "Funding Information: Funded by the Heart and Stroke Foundation of Ontario and the Natural Sciences and Engineering Research Council of Canada. Publisher Copyright: {\textcopyright} 1999 American Society of Mechanical Engineers (ASME). All rights reserved.; ASME 1999 International Mechanical Engineering Congress and Exposition, IMECE 1999 ; Conference date: 14-11-1999 Through 19-11-1999",

year = "1999",

doi = "10.1115/IMECE1999-0392",

language = "English",

series = "ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)",

publisher = "American Society of Mechanical Engineers (ASME)",

pages = "91--92",

booktitle = "Advances in Bioengineering",

}

TY - GEN

T1 - THERMOMECHANICAL CHARACTERIZATION OF COLLAGEN CROSSLINKING IN DEVELOPING CARDIOVASCULAR TISSUES

AU - Wells, Sarah M.

AU - Naimark, Wendy A.

AU - Lee, J. Michael

N1 - Funding Information: Funded by the Heart and Stroke Foundation of Ontario and the Natural Sciences and Engineering Research Council of Canada. Publisher Copyright: © 1999 American Society of Mechanical Engineers (ASME). All rights reserved.

PY - 1999

Y1 - 1999

N2 - We have used hydrothermal isometric tension (HIT) tests to assess developmental changes in collagen crosslinking in the ovine thoracic aorta and pericardium. Tissue strips were loaded under isometric tension and heated to 90°C for 3 lirs. Load relaxation at this temperature is associated with collagen hydrolysis and chain slippage, the half-time of load decay (t1/2) being an indicator of collagen crosslinking. t1/2was computed before and after tissues were treated with NaBH4which stabilizes immature crosslinks. The t1/2of untreated tissue increased from the lamb to the adult, indicating that collagen crosslinking increased postnatally. Furthermore, the t1/2of NaBH4-treated lamb tissue (3d pericardium and 21d aorta) was similar to that of untreated adult tissue, suggesting that much immature crosslinking in the lamb is stabilized postnatally. These observations suggest (i) increased crosslinking occurs postnatally, (ii) this increase is largely due to the conversion of immature crosslinks into their mature, heat stable form (Naimark et al., 1998, Wells et al., 1999).

AB - We have used hydrothermal isometric tension (HIT) tests to assess developmental changes in collagen crosslinking in the ovine thoracic aorta and pericardium. Tissue strips were loaded under isometric tension and heated to 90°C for 3 lirs. Load relaxation at this temperature is associated with collagen hydrolysis and chain slippage, the half-time of load decay (t1/2) being an indicator of collagen crosslinking. t1/2was computed before and after tissues were treated with NaBH4which stabilizes immature crosslinks. The t1/2of untreated tissue increased from the lamb to the adult, indicating that collagen crosslinking increased postnatally. Furthermore, the t1/2of NaBH4-treated lamb tissue (3d pericardium and 21d aorta) was similar to that of untreated adult tissue, suggesting that much immature crosslinking in the lamb is stabilized postnatally. These observations suggest (i) increased crosslinking occurs postnatally, (ii) this increase is largely due to the conversion of immature crosslinks into their mature, heat stable form (Naimark et al., 1998, Wells et al., 1999).

UR - http://www.scopus.com/inward/record.url?scp=85122667153&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85122667153&partnerID=8YFLogxK

U2 - 10.1115/IMECE1999-0392

DO - 10.1115/IMECE1999-0392

M3 - Conference contribution

AN - SCOPUS:85122667153

T3 - ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)

SP - 91

EP - 92

BT - Advances in Bioengineering

PB - American Society of Mechanical Engineers (ASME)

T2 - ASME 1999 International Mechanical Engineering Congress and Exposition, IMECE 1999

Y2 - 14 November 1999 through 19 November 1999

ER -

THERMOMECHANICAL CHARACTERIZATION OF COLLAGEN CROSSLINKING IN DEVELOPING CARDIOVASCULAR TISSUES

Resumen

Serie de la publicación

Conference

Nota bibliográfica

ASJC Scopus Subject Areas

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto