THERMOMECHANICAL CHARACTERIZATION OF COLLAGEN CROSSLINKING IN DEVELOPING CARDIOVASCULAR TISSUES

Sarah M. Wells; Wendy A. Naimark; J. Michael Lee

doi:10.1115/IMECE1999-0392

THERMOMECHANICAL CHARACTERIZATION OF COLLAGEN CROSSLINKING IN DEVELOPING CARDIOVASCULAR TISSUES

Sarah M. Wells, Wendy A. Naimark, J. Michael Lee

Medicine

Medicine

Résultat de recherche: Conference contribution

Résumé

We have used hydrothermal isometric tension (HIT) tests to assess developmental changes in collagen crosslinking in the ovine thoracic aorta and pericardium. Tissue strips were loaded under isometric tension and heated to 90°C for 3 lirs. Load relaxation at this temperature is associated with collagen hydrolysis and chain slippage, the half-time of load decay (t_1/2) being an indicator of collagen crosslinking. t_1/2was computed before and after tissues were treated with NaBH₄which stabilizes immature crosslinks. The t_1/2of untreated tissue increased from the lamb to the adult, indicating that collagen crosslinking increased postnatally. Furthermore, the t_1/2of NaBH₄-treated lamb tissue (3d pericardium and 21d aorta) was similar to that of untreated adult tissue, suggesting that much immature crosslinking in the lamb is stabilized postnatally. These observations suggest (i) increased crosslinking occurs postnatally, (ii) this increase is largely due to the conversion of immature crosslinks into their mature, heat stable form (Naimark et al., 1998, Wells et al., 1999).

Langue d'origine	English
Titre de la publication principale	Advances in Bioengineering
Maison d'édition	American Society of Mechanical Engineers (ASME)
Pages	91-92
Nombre de pages	2
ISBN (électronique)	9780791816400
DOI	https://doi.org/10.1115/IMECE1999-0392
Statut de publication	Published - 1999
Événement	ASME 1999 International Mechanical Engineering Congress and Exposition, IMECE 1999 - Nashville, United States Durée: nov. 14 1999 → nov. 19 1999

Séries de publication

Prénom	ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)
Volume	1999-D

Conference

Conference	ASME 1999 International Mechanical Engineering Congress and Exposition, IMECE 1999
Pays/Territoire	United States
Ville	Nashville
Période	11/14/99 → 11/19/99

Note bibliographique

Funding Information:
Funded by the Heart and Stroke Foundation of Ontario and the Natural Sciences and Engineering Research Council of Canada.

Publisher Copyright:
© 1999 American Society of Mechanical Engineers (ASME). All rights reserved.

ASJC Scopus Subject Areas

Mechanical Engineering

Accès au document

10.1115/IMECE1999-0392

Autres fichiers et liens

Citer

Wells, S. M., Naimark, W. A., & Lee, J. M. (1999). THERMOMECHANICAL CHARACTERIZATION OF COLLAGEN CROSSLINKING IN DEVELOPING CARDIOVASCULAR TISSUES. Dans Advances in Bioengineering (pp. 91-92). (ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE); Vol. 1999-D). American Society of Mechanical Engineers (ASME). https://doi.org/10.1115/IMECE1999-0392

THERMOMECHANICAL CHARACTERIZATION OF COLLAGEN CROSSLINKING IN DEVELOPING CARDIOVASCULAR TISSUES. / Wells, Sarah M.; Naimark, Wendy A.; Lee, J. Michael.
Advances in Bioengineering. American Society of Mechanical Engineers (ASME), 1999. p. 91-92 (ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE); Vol. 1999-D).

Résultat de recherche: Conference contribution

Wells, SM, Naimark, WA & Lee, JM 1999, THERMOMECHANICAL CHARACTERIZATION OF COLLAGEN CROSSLINKING IN DEVELOPING CARDIOVASCULAR TISSUES. dans Advances in Bioengineering. ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE), vol. 1999-D, American Society of Mechanical Engineers (ASME), pp. 91-92, ASME 1999 International Mechanical Engineering Congress and Exposition, IMECE 1999, Nashville, United States, 11/14/99. https://doi.org/10.1115/IMECE1999-0392

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abstract = "We have used hydrothermal isometric tension (HIT) tests to assess developmental changes in collagen crosslinking in the ovine thoracic aorta and pericardium. Tissue strips were loaded under isometric tension and heated to 90°C for 3 lirs. Load relaxation at this temperature is associated with collagen hydrolysis and chain slippage, the half-time of load decay (t1/2) being an indicator of collagen crosslinking. t1/2was computed before and after tissues were treated with NaBH4which stabilizes immature crosslinks. The t1/2of untreated tissue increased from the lamb to the adult, indicating that collagen crosslinking increased postnatally. Furthermore, the t1/2of NaBH4-treated lamb tissue (3d pericardium and 21d aorta) was similar to that of untreated adult tissue, suggesting that much immature crosslinking in the lamb is stabilized postnatally. These observations suggest (i) increased crosslinking occurs postnatally, (ii) this increase is largely due to the conversion of immature crosslinks into their mature, heat stable form (Naimark et al., 1998, Wells et al., 1999).",

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N2 - We have used hydrothermal isometric tension (HIT) tests to assess developmental changes in collagen crosslinking in the ovine thoracic aorta and pericardium. Tissue strips were loaded under isometric tension and heated to 90°C for 3 lirs. Load relaxation at this temperature is associated with collagen hydrolysis and chain slippage, the half-time of load decay (t1/2) being an indicator of collagen crosslinking. t1/2was computed before and after tissues were treated with NaBH4which stabilizes immature crosslinks. The t1/2of untreated tissue increased from the lamb to the adult, indicating that collagen crosslinking increased postnatally. Furthermore, the t1/2of NaBH4-treated lamb tissue (3d pericardium and 21d aorta) was similar to that of untreated adult tissue, suggesting that much immature crosslinking in the lamb is stabilized postnatally. These observations suggest (i) increased crosslinking occurs postnatally, (ii) this increase is largely due to the conversion of immature crosslinks into their mature, heat stable form (Naimark et al., 1998, Wells et al., 1999).

AB - We have used hydrothermal isometric tension (HIT) tests to assess developmental changes in collagen crosslinking in the ovine thoracic aorta and pericardium. Tissue strips were loaded under isometric tension and heated to 90°C for 3 lirs. Load relaxation at this temperature is associated with collagen hydrolysis and chain slippage, the half-time of load decay (t1/2) being an indicator of collagen crosslinking. t1/2was computed before and after tissues were treated with NaBH4which stabilizes immature crosslinks. The t1/2of untreated tissue increased from the lamb to the adult, indicating that collagen crosslinking increased postnatally. Furthermore, the t1/2of NaBH4-treated lamb tissue (3d pericardium and 21d aorta) was similar to that of untreated adult tissue, suggesting that much immature crosslinking in the lamb is stabilized postnatally. These observations suggest (i) increased crosslinking occurs postnatally, (ii) this increase is largely due to the conversion of immature crosslinks into their mature, heat stable form (Naimark et al., 1998, Wells et al., 1999).

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THERMOMECHANICAL CHARACTERIZATION OF COLLAGEN CROSSLINKING IN DEVELOPING CARDIOVASCULAR TISSUES

Résumé

Séries de publication

Conference

Note bibliographique

ASJC Scopus Subject Areas

Accès au document

Autres fichiers et liens

Empreinte numérique

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