Uracil-DNA glycosylase (UNG) influences the melting temperature (Tm) of herpes simplex virus (HSV) hybridization probes

Jason J. LeBlanc; Janice Pettipas; Sarah J. Campbell; Ross J. Davidson; Todd F. Hatchette

doi:10.1016/j.jviromet.2008.03.024

Uracil-DNA glycosylase (UNG) influences the melting temperature (T_m) of herpes simplex virus (HSV) hybridization probes

Jason J. LeBlanc, Janice Pettipas, Sarah J. Campbell, Ross J. Davidson, Todd F. Hatchette

Medicine

Medicine

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

10 Citas (Scopus)

Resumen

Prior to PCR amplification, uracil-DNA glycosylase (UNG) can be incorporated to prevent amplicon contamination. Melting temperature (T_m) analysis of herpes simplex virus (HSV) hybridization probes was used to demonstrate a concentration-dependent decrease in T_m values when heat stabile or heat labile UNG was added.

Idioma original	English
Páginas (desde-hasta)	158-160
Número de páginas	3
Publicación	Journal of Virological Methods
Volumen	151
N.º	1
DOI	https://doi.org/10.1016/j.jviromet.2008.03.024
Estado	Published - jul. 2008

ASJC Scopus Subject Areas

Virology

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10.1016/j.jviromet.2008.03.024

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abstract = "Prior to PCR amplification, uracil-DNA glycosylase (UNG) can be incorporated to prevent amplicon contamination. Melting temperature (Tm) analysis of herpes simplex virus (HSV) hybridization probes was used to demonstrate a concentration-dependent decrease in Tm values when heat stabile or heat labile UNG was added.",

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AU - Davidson, Ross J.

AU - Hatchette, Todd F.

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