Uracil-DNA glycosylase (UNG) influences the melting temperature (Tm) of herpes simplex virus (HSV) hybridization probes

Jason J. LeBlanc; Janice Pettipas; Sarah J. Campbell; Ross J. Davidson; Todd F. Hatchette

doi:10.1016/j.jviromet.2008.03.024

Uracil-DNA glycosylase (UNG) influences the melting temperature (T_m) of herpes simplex virus (HSV) hybridization probes

Jason J. LeBlanc, Janice Pettipas, Sarah J. Campbell, Ross J. Davidson, Todd F. Hatchette

Medicine

Medicine

Résultat de recherche: Article › examen par les pairs

10 Citations (Scopus)

Résumé

Prior to PCR amplification, uracil-DNA glycosylase (UNG) can be incorporated to prevent amplicon contamination. Melting temperature (T_m) analysis of herpes simplex virus (HSV) hybridization probes was used to demonstrate a concentration-dependent decrease in T_m values when heat stabile or heat labile UNG was added.

Langue d'origine	English
Pages (de-à)	158-160
Nombre de pages	3
Journal	Journal of Virological Methods
Volume	151
Numéro de publication	1
DOI	https://doi.org/10.1016/j.jviromet.2008.03.024
Statut de publication	Published - juill. 2008

ASJC Scopus Subject Areas

Virology

Accès au document

10.1016/j.jviromet.2008.03.024

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title = "Uracil-DNA glycosylase (UNG) influences the melting temperature (Tm) of herpes simplex virus (HSV) hybridization probes",

abstract = "Prior to PCR amplification, uracil-DNA glycosylase (UNG) can be incorporated to prevent amplicon contamination. Melting temperature (Tm) analysis of herpes simplex virus (HSV) hybridization probes was used to demonstrate a concentration-dependent decrease in Tm values when heat stabile or heat labile UNG was added.",

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AU - Davidson, Ross J.

AU - Hatchette, Todd F.

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