TY - JOUR
T1 - Chemokine production and adhesion molecule expression by neural cells exposed to IL-1, TNFα and interferon
AU - Chuluyan, H. Eduardo
AU - Lang, Bianca J.
AU - Yoshimura, Teizo
AU - Kenney, John S.
AU - Issekutz, Andrew C.
PY - 1998/10/16
Y1 - 1998/10/16
N2 - We investigated the effect of TNFα, IL-1α and IFNγ on two neuroblastoma (NB) cell lines (SK-N-SH and SK-N-MC). These lines responded differentially to IL-1α, TNFα and IFNγ for MCP-1 and IL-8 production and expression of the ICAM-1 and VCAM-1 adhesion molecules. None of the cytokines induced MCP-1 or 1L-8 on SK-N-MC cells. Both chemokines were produced in response to IL-1α by SK-N-SH cells, while TNFα induced mainly MCP-1 production. Addition of IFNγ decreased IL-8, but not MCP-1 production. These responses correlated with monocyte and neutrophil chemotactic activity in NB culture supernatants. This activity was neutralized by antibodies to IL-8 and MCP-1. The expression of ICAM-1 on SK-N-MC was up-regulated by TNFα or IFNγ, while IL-1α also up-regulated ICAM-1 on SK-N-SH cells. VCAM-1 expression on SK-N-SH was induced by IL-1α and TNFα and IFNγ synergized with TNFα in this respect on both NB cell lines. These results suggest that mechanisms for chemokine production and VCAM-1 and ICAM-1 up-regulation by inflammatory cytokines differ and IFNγ, in conjunction with TNFα, stimulate neural cell responses (high MCP-1 and VCAM-1 and decreased IL-8) favouring mononuclear cell recruitment.
AB - We investigated the effect of TNFα, IL-1α and IFNγ on two neuroblastoma (NB) cell lines (SK-N-SH and SK-N-MC). These lines responded differentially to IL-1α, TNFα and IFNγ for MCP-1 and IL-8 production and expression of the ICAM-1 and VCAM-1 adhesion molecules. None of the cytokines induced MCP-1 or 1L-8 on SK-N-MC cells. Both chemokines were produced in response to IL-1α by SK-N-SH cells, while TNFα induced mainly MCP-1 production. Addition of IFNγ decreased IL-8, but not MCP-1 production. These responses correlated with monocyte and neutrophil chemotactic activity in NB culture supernatants. This activity was neutralized by antibodies to IL-8 and MCP-1. The expression of ICAM-1 on SK-N-MC was up-regulated by TNFα or IFNγ, while IL-1α also up-regulated ICAM-1 on SK-N-SH cells. VCAM-1 expression on SK-N-SH was induced by IL-1α and TNFα and IFNγ synergized with TNFα in this respect on both NB cell lines. These results suggest that mechanisms for chemokine production and VCAM-1 and ICAM-1 up-regulation by inflammatory cytokines differ and IFNγ, in conjunction with TNFα, stimulate neural cell responses (high MCP-1 and VCAM-1 and decreased IL-8) favouring mononuclear cell recruitment.
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U2 - 10.1016/S0024-3205(98)00470-6
DO - 10.1016/S0024-3205(98)00470-6
M3 - Article
C2 - 9825772
AN - SCOPUS:0032538389
SN - 0024-3205
VL - 63
SP - 1939
EP - 1952
JO - Life Sciences
JF - Life Sciences
IS - 21
ER -