Cryo-EM Structure of an Extended SARS-CoV-2 Replication and Transcription Complex Reveals an Intermediate State in Cap Synthesis

Liming Yan, Ji Ge, Litao Zheng, Ying Zhang, Yan Gao, Tao Wang, Yucen Huang, Yunxiang Yang, Shan Gao, Mingyu Li, Zhenyu Liu, Haofeng Wang, Yingjian Li, Yu Chen, Luke W. Guddat, Quan Wang, Zihe Rao, Zhiyong Lou

Research output: Contribution to journalArticlepeer-review

196 Citations (Scopus)

Abstract

Yan et al. present a cryo-EM structure of the SARS-CoV-2 replication and transcription complex that includes the single RNA-binding protein nsp9. The structural snapshot provides insight into how the viral machinery catalyzes a crucial step in viral mRNA cap synthesis, which is important for virus survival.

Original languageEnglish
Pages (from-to)184-193.e10
JournalCell
Volume184
Issue number1
DOIs
Publication statusPublished - Jan 7 2021
Externally publishedYes

Bibliographical note

Funding Information:
We thank Prof. Haiteng Deng and Dr. Meng Han of Tsinghua University for their assistance for mass spectrum analysis. We also must express our gratitude to the Bio-Electron Microscopy Facility of ShanghaiTech University for data collection. This work was supported by the National Program on Key Research Project of China ( 2020YFA0707500 and 2017YFC0840300 ) and Tsinghua University Spring Breeze Fund .

Funding Information:
We thank Prof. Haiteng Deng and Dr. Meng Han of Tsinghua University for their assistance for mass spectrum analysis. We also must express our gratitude to the Bio-Electron Microscopy Facility of ShanghaiTech University for data collection. This work was supported by the National Program on Key Research Project of China (2020YFA0707500 and 2017YFC0840300) and Tsinghua University Spring Breeze Fund. Z.R. and Z. Lou conceived the project and designed the experiments. L.Y. J.G. L.Z. Y.Z. Y.G. T.W. Y.Y. S.G. M.L. Z. Liu, H.W. and Y.L. performed experiments. L.Y. Y.H. Y.C. Q.W. Z. Lou, and Z.R. analyzed the data. Z.R. Z. Lou, L.Y. and L.W.G. wrote the manuscript. All authors discussed the experiments and read and approved the manuscript. The authors declare no competing interests.

Publisher Copyright:
© 2020 Elsevier Inc.

ASJC Scopus Subject Areas

  • General Biochemistry,Genetics and Molecular Biology

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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Yan, L., Ge, J., Zheng, L., Zhang, Y., Gao, Y., Wang, T., Huang, Y., Yang, Y., Gao, S., Li, M., Liu, Z., Wang, H., Li, Y., Chen, Y., Guddat, L. W., Wang, Q., Rao, Z., & Lou, Z. (2021). Cryo-EM Structure of an Extended SARS-CoV-2 Replication and Transcription Complex Reveals an Intermediate State in Cap Synthesis. Cell, 184(1), 184-193.e10. https://doi.org/10.1016/j.cell.2020.11.016