Cryo-EM Structure of an Extended SARS-CoV-2 Replication and Transcription Complex Reveals an Intermediate State in Cap Synthesis

Liming Yan; Ji Ge; Litao Zheng; Ying Zhang; Yan Gao; Tao Wang; Yucen Huang; Yunxiang Yang; Shan Gao; Mingyu Li; Zhenyu Liu; Haofeng Wang; Yingjian Li; Yu Chen; Luke W. Guddat; Quan Wang; Zihe Rao; Zhiyong Lou

doi:10.1016/j.cell.2020.11.016

Cryo-EM Structure of an Extended SARS-CoV-2 Replication and Transcription Complex Reveals an Intermediate State in Cap Synthesis

Liming Yan, Ji Ge, Litao Zheng, Ying Zhang, Yan Gao, Tao Wang, Yucen Huang, Yunxiang Yang, Shan Gao, Mingyu Li, Zhenyu Liu, Haofeng Wang, Yingjian Li, Yu Chen, Luke W. Guddat, Quan Wang, Zihe Rao, Zhiyong Lou

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

194 Citas (Scopus)

Resumen

Yan et al. present a cryo-EM structure of the SARS-CoV-2 replication and transcription complex that includes the single RNA-binding protein nsp9. The structural snapshot provides insight into how the viral machinery catalyzes a crucial step in viral mRNA cap synthesis, which is important for virus survival.

Idioma original	English
Páginas (desde-hasta)	184-193.e10
Publicación	Cell
Volumen	184
N.º	1
DOI	https://doi.org/10.1016/j.cell.2020.11.016
Estado	Published - ene. 7 2021
Publicado de forma externa	Sí

Nota bibliográfica

Funding Information:
We thank Prof. Haiteng Deng and Dr. Meng Han of Tsinghua University for their assistance for mass spectrum analysis. We also must express our gratitude to the Bio-Electron Microscopy Facility of ShanghaiTech University for data collection. This work was supported by the National Program on Key Research Project of China ( 2020YFA0707500 and 2017YFC0840300 ) and Tsinghua University Spring Breeze Fund .

Funding Information:
We thank Prof. Haiteng Deng and Dr. Meng Han of Tsinghua University for their assistance for mass spectrum analysis. We also must express our gratitude to the Bio-Electron Microscopy Facility of ShanghaiTech University for data collection. This work was supported by the National Program on Key Research Project of China (2020YFA0707500 and 2017YFC0840300) and Tsinghua University Spring Breeze Fund. Z.R. and Z. Lou conceived the project and designed the experiments. L.Y. J.G. L.Z. Y.Z. Y.G. T.W. Y.Y. S.G. M.L. Z. Liu, H.W. and Y.L. performed experiments. L.Y. Y.H. Y.C. Q.W. Z. Lou, and Z.R. analyzed the data. Z.R. Z. Lou, L.Y. and L.W.G. wrote the manuscript. All authors discussed the experiments and read and approved the manuscript. The authors declare no competing interests.

Publisher Copyright:
© 2020 Elsevier Inc.

ASJC Scopus Subject Areas

General Biochemistry,Genetics and Molecular Biology

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

Acceder al documento

10.1016/j.cell.2020.11.016

Otros archivos y enlaces

Citar esto

Yan, L., Ge, J., Zheng, L., Zhang, Y., Gao, Y., Wang, T., Huang, Y., Yang, Y., Gao, S., Li, M., Liu, Z., Wang, H., Li, Y., Chen, Y., Guddat, L. W., Wang, Q., Rao, Z., & Lou, Z. (2021). Cryo-EM Structure of an Extended SARS-CoV-2 Replication and Transcription Complex Reveals an Intermediate State in Cap Synthesis. Cell, 184(1), 184-193.e10. https://doi.org/10.1016/j.cell.2020.11.016

Yan, L, Ge, J, Zheng, L, Zhang, Y, Gao, Y, Wang, T, Huang, Y, Yang, Y, Gao, S, Li, M, Liu, Z, Wang, H, Li, Y, Chen, Y, Guddat, LW, Wang, Q, Rao, Z & Lou, Z 2021, 'Cryo-EM Structure of an Extended SARS-CoV-2 Replication and Transcription Complex Reveals an Intermediate State in Cap Synthesis', Cell, vol. 184, n.º 1, pp. 184-193.e10. https://doi.org/10.1016/j.cell.2020.11.016

@article{11027fe3e7764db8aa97f53eb42024cb,

title = "Cryo-EM Structure of an Extended SARS-CoV-2 Replication and Transcription Complex Reveals an Intermediate State in Cap Synthesis",

abstract = "Yan et al. present a cryo-EM structure of the SARS-CoV-2 replication and transcription complex that includes the single RNA-binding protein nsp9. The structural snapshot provides insight into how the viral machinery catalyzes a crucial step in viral mRNA cap synthesis, which is important for virus survival.",

author = "Liming Yan and Ji Ge and Litao Zheng and Ying Zhang and Yan Gao and Tao Wang and Yucen Huang and Yunxiang Yang and Shan Gao and Mingyu Li and Zhenyu Liu and Haofeng Wang and Yingjian Li and Yu Chen and Guddat, {Luke W.} and Quan Wang and Zihe Rao and Zhiyong Lou",

note = "Funding Information: We thank Prof. Haiteng Deng and Dr. Meng Han of Tsinghua University for their assistance for mass spectrum analysis. We also must express our gratitude to the Bio-Electron Microscopy Facility of ShanghaiTech University for data collection. This work was supported by the National Program on Key Research Project of China ( 2020YFA0707500 and 2017YFC0840300 ) and Tsinghua University Spring Breeze Fund . Funding Information: We thank Prof. Haiteng Deng and Dr. Meng Han of Tsinghua University for their assistance for mass spectrum analysis. We also must express our gratitude to the Bio-Electron Microscopy Facility of ShanghaiTech University for data collection. This work was supported by the National Program on Key Research Project of China (2020YFA0707500 and 2017YFC0840300) and Tsinghua University Spring Breeze Fund. Z.R. and Z. Lou conceived the project and designed the experiments. L.Y. J.G. L.Z. Y.Z. Y.G. T.W. Y.Y. S.G. M.L. Z. Liu, H.W. and Y.L. performed experiments. L.Y. Y.H. Y.C. Q.W. Z. Lou, and Z.R. analyzed the data. Z.R. Z. Lou, L.Y. and L.W.G. wrote the manuscript. All authors discussed the experiments and read and approved the manuscript. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",

year = "2021",

month = jan,

day = "7",

doi = "10.1016/j.cell.2020.11.016",

language = "English",

volume = "184",

pages = "184--193.e10",

journal = "Cell",

issn = "0092-8674",

publisher = "Cell Press",

number = "1",

}

TY - JOUR

T1 - Cryo-EM Structure of an Extended SARS-CoV-2 Replication and Transcription Complex Reveals an Intermediate State in Cap Synthesis

AU - Yan, Liming

AU - Ge, Ji

AU - Zheng, Litao

AU - Zhang, Ying

AU - Gao, Yan

AU - Wang, Tao

AU - Huang, Yucen

AU - Yang, Yunxiang

AU - Gao, Shan

AU - Li, Mingyu

AU - Liu, Zhenyu

AU - Wang, Haofeng

AU - Li, Yingjian

AU - Chen, Yu

AU - Guddat, Luke W.

AU - Wang, Quan

AU - Rao, Zihe

AU - Lou, Zhiyong

N1 - Funding Information: We thank Prof. Haiteng Deng and Dr. Meng Han of Tsinghua University for their assistance for mass spectrum analysis. We also must express our gratitude to the Bio-Electron Microscopy Facility of ShanghaiTech University for data collection. This work was supported by the National Program on Key Research Project of China ( 2020YFA0707500 and 2017YFC0840300 ) and Tsinghua University Spring Breeze Fund . Funding Information: We thank Prof. Haiteng Deng and Dr. Meng Han of Tsinghua University for their assistance for mass spectrum analysis. We also must express our gratitude to the Bio-Electron Microscopy Facility of ShanghaiTech University for data collection. This work was supported by the National Program on Key Research Project of China (2020YFA0707500 and 2017YFC0840300) and Tsinghua University Spring Breeze Fund. Z.R. and Z. Lou conceived the project and designed the experiments. L.Y. J.G. L.Z. Y.Z. Y.G. T.W. Y.Y. S.G. M.L. Z. Liu, H.W. and Y.L. performed experiments. L.Y. Y.H. Y.C. Q.W. Z. Lou, and Z.R. analyzed the data. Z.R. Z. Lou, L.Y. and L.W.G. wrote the manuscript. All authors discussed the experiments and read and approved the manuscript. The authors declare no competing interests. Publisher Copyright: © 2020 Elsevier Inc.

PY - 2021/1/7

Y1 - 2021/1/7

N2 - Yan et al. present a cryo-EM structure of the SARS-CoV-2 replication and transcription complex that includes the single RNA-binding protein nsp9. The structural snapshot provides insight into how the viral machinery catalyzes a crucial step in viral mRNA cap synthesis, which is important for virus survival.

AB - Yan et al. present a cryo-EM structure of the SARS-CoV-2 replication and transcription complex that includes the single RNA-binding protein nsp9. The structural snapshot provides insight into how the viral machinery catalyzes a crucial step in viral mRNA cap synthesis, which is important for virus survival.

UR - http://www.scopus.com/inward/record.url?scp=85096517151&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85096517151&partnerID=8YFLogxK

U2 - 10.1016/j.cell.2020.11.016

DO - 10.1016/j.cell.2020.11.016

M3 - Article

C2 - 33232691

AN - SCOPUS:85096517151

SN - 0092-8674

VL - 184

SP - 184-193.e10

JO - Cell

JF - Cell

IS - 1

ER -

Cryo-EM Structure of an Extended SARS-CoV-2 Replication and Transcription Complex Reveals an Intermediate State in Cap Synthesis

Resumen

Nota bibliográfica

ASJC Scopus Subject Areas

PubMed: MeSH publication types

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto