Multiplexed Quantitative Proteomic Profiling of Cancer Cells and Tissues Using Isobaric Labeling-Based Tags

Mukhayyo Sultonova; Devanand M. Pinto; J. Patrick Murphy

doi:10.1007/978-1-0716-2376-3_16

Multiplexed Quantitative Proteomic Profiling of Cancer Cells and Tissues Using Isobaric Labeling-Based Tags

Mukhayyo Sultonova, Devanand M. Pinto, J. Patrick Murphy

Research output: Contribution to journal › Article › peer-review

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Abstract

Comparing cancer proteomes across many samples offers a window into cancer cell biology and may reveal new treatment options for specific subsets of cancer. Here we describe a method using tandem mass tag (TMT) technology to multiplex up to 18 samples in a single analysis, paving the way for the analysis of large cohorts of tumors, cell lines, and perturbations thereof. The procedure we describe will result in samples ready for in-depth LC-MS/MS analysis in 3-4 days.

Original language	English
Pages (from-to)	211-223
Number of pages	13
Journal	Methods in Molecular Biology
Volume	2508
DOIs	https://doi.org/10.1007/978-1-0716-2376-3_16
Publication status	Published - 2022
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

ASJC Scopus Subject Areas

Molecular Biology
Genetics

PubMed: MeSH publication types

Journal Article

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Cite this

Sultonova, M., Pinto, D. M., & Murphy, J. P. (2022). Multiplexed Quantitative Proteomic Profiling of Cancer Cells and Tissues Using Isobaric Labeling-Based Tags. Methods in Molecular Biology, 2508, 211-223. https://doi.org/10.1007/978-1-0716-2376-3_16

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Multiplexed Quantitative Proteomic Profiling of Cancer Cells and Tissues Using Isobaric Labeling-Based Tags

Abstract

Bibliographical note

ASJC Scopus Subject Areas

PubMed: MeSH publication types

UN SDGs

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