Multiplexed Quantitative Proteomic Profiling of Cancer Cells and Tissues Using Isobaric Labeling-Based Tags

Mukhayyo Sultonova; Devanand M. Pinto; J. Patrick Murphy

doi:10.1007/978-1-0716-2376-3_16

Multiplexed Quantitative Proteomic Profiling of Cancer Cells and Tissues Using Isobaric Labeling-Based Tags

Mukhayyo Sultonova, Devanand M. Pinto, J. Patrick Murphy

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

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Resumen

Comparing cancer proteomes across many samples offers a window into cancer cell biology and may reveal new treatment options for specific subsets of cancer. Here we describe a method using tandem mass tag (TMT) technology to multiplex up to 18 samples in a single analysis, paving the way for the analysis of large cohorts of tumors, cell lines, and perturbations thereof. The procedure we describe will result in samples ready for in-depth LC-MS/MS analysis in 3-4 days.

Idioma original	English
Páginas (desde-hasta)	211-223
Número de páginas	13
Publicación	Methods in Molecular Biology
Volumen	2508
DOI	https://doi.org/10.1007/978-1-0716-2376-3_16
Estado	Published - 2022
Publicado de forma externa	Sí

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Publisher Copyright:
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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Molecular Biology
Genetics

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JF - Methods in Molecular Biology

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Multiplexed Quantitative Proteomic Profiling of Cancer Cells and Tissues Using Isobaric Labeling-Based Tags

Resumen

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ASJC Scopus Subject Areas

PubMed: MeSH publication types

ODS de las Naciones Unidas

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