Multiplexed Quantitative Proteomic Profiling of Cancer Cells and Tissues Using Isobaric Labeling-Based Tags

Mukhayyo Sultonova, Devanand M. Pinto, J. Patrick Murphy

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1 Citation (Scopus)

Résumé

Comparing cancer proteomes across many samples offers a window into cancer cell biology and may reveal new treatment options for specific subsets of cancer. Here we describe a method using tandem mass tag (TMT) technology to multiplex up to 18 samples in a single analysis, paving the way for the analysis of large cohorts of tumors, cell lines, and perturbations thereof. The procedure we describe will result in samples ready for in-depth LC-MS/MS analysis in 3-4 days.

Langue d'origineEnglish
Pages (de-à)211-223
Nombre de pages13
JournalMethods in Molecular Biology
Volume2508
DOI
Statut de publicationPublished - 2022
Publié à l'externeOui

Note bibliographique

Publisher Copyright:
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

ASJC Scopus Subject Areas

  • Molecular Biology
  • Genetics

PubMed: MeSH publication types

  • Journal Article

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