Multiplexed Quantitative Proteomic Profiling of Cancer Cells and Tissues Using Isobaric Labeling-Based Tags

Mukhayyo Sultonova; Devanand M. Pinto; J. Patrick Murphy

doi:10.1007/978-1-0716-2376-3_16

Multiplexed Quantitative Proteomic Profiling of Cancer Cells and Tissues Using Isobaric Labeling-Based Tags

Mukhayyo Sultonova, Devanand M. Pinto, J. Patrick Murphy

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1 Citation (Scopus)

Résumé

Comparing cancer proteomes across many samples offers a window into cancer cell biology and may reveal new treatment options for specific subsets of cancer. Here we describe a method using tandem mass tag (TMT) technology to multiplex up to 18 samples in a single analysis, paving the way for the analysis of large cohorts of tumors, cell lines, and perturbations thereof. The procedure we describe will result in samples ready for in-depth LC-MS/MS analysis in 3-4 days.

Langue d'origine	English
Pages (de-à)	211-223
Nombre de pages	13
Journal	Methods in Molecular Biology
Volume	2508
DOI	https://doi.org/10.1007/978-1-0716-2376-3_16
Statut de publication	Published - 2022
Publié à l'externe	Oui

Note bibliographique

Publisher Copyright:
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

ASJC Scopus Subject Areas

Molecular Biology
Genetics

PubMed: MeSH publication types

Journal Article

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Cette action contribue à la réalisation des objectifs de développement durable suivants

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10.1007/978-1-0716-2376-3_16

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abstract = "Comparing cancer proteomes across many samples offers a window into cancer cell biology and may reveal new treatment options for specific subsets of cancer. Here we describe a method using tandem mass tag (TMT) technology to multiplex up to 18 samples in a single analysis, paving the way for the analysis of large cohorts of tumors, cell lines, and perturbations thereof. The procedure we describe will result in samples ready for in-depth LC-MS/MS analysis in 3-4 days.",

author = "Mukhayyo Sultonova and Pinto, {Devanand M.} and Murphy, {J. Patrick}",

note = "Publisher Copyright: {\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2022",

doi = "10.1007/978-1-0716-2376-3_16",

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AU - Sultonova, Mukhayyo

AU - Pinto, Devanand M.

AU - Murphy, J. Patrick

PY - 2022

Y1 - 2022

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AB - Comparing cancer proteomes across many samples offers a window into cancer cell biology and may reveal new treatment options for specific subsets of cancer. Here we describe a method using tandem mass tag (TMT) technology to multiplex up to 18 samples in a single analysis, paving the way for the analysis of large cohorts of tumors, cell lines, and perturbations thereof. The procedure we describe will result in samples ready for in-depth LC-MS/MS analysis in 3-4 days.

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JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Multiplexed Quantitative Proteomic Profiling of Cancer Cells and Tissues Using Isobaric Labeling-Based Tags

Résumé

Note bibliographique

ASJC Scopus Subject Areas

PubMed: MeSH publication types

ODD de l’ONU

Accès au document

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