Single-Cell RNA Sequencing Analysis Using Fluidigm C1 Platform for Characterization of Heterogeneous Transcriptomes

Jinhong Kim; Paola A. Marignani

doi:10.1007/978-1-0716-2376-3_19

Single-Cell RNA Sequencing Analysis Using Fluidigm C1 Platform for Characterization of Heterogeneous Transcriptomes

Jinhong Kim, Paola A. Marignani

Medicine

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Single-cell RNA sequencing (scRNA-seq) is an innovative technology that can be used to characterize transcriptome heterogeneity at the single-cell resolution. The Fluidigm C1 is an automated system for preparing complementary deoxyribonucleic acid (cDNA) samples that have been synthesized and preamplified from single cells for next-generation sequencing (NGS) analysis. Herein, we detail a workflow and protocol for scRNA-seq analysis of 3'-end enriched cDNA libraries and introduce strategies for validation of differential gene expression.

Original language	English
Pages (from-to)	261-278
Number of pages	18
Journal	Methods in Molecular Biology
Volume	2508
DOIs	https://doi.org/10.1007/978-1-0716-2376-3_19
Publication status	Published - 2022

Bibliographical note

Publisher Copyright:
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

ASJC Scopus Subject Areas

Molecular Biology
Genetics

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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Cite this

Kim, J., & Marignani, P. A. (2022). Single-Cell RNA Sequencing Analysis Using Fluidigm C1 Platform for Characterization of Heterogeneous Transcriptomes. Methods in Molecular Biology, 2508, 261-278. https://doi.org/10.1007/978-1-0716-2376-3_19

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Single-Cell RNA Sequencing Analysis Using Fluidigm C1 Platform for Characterization of Heterogeneous Transcriptomes

Abstract

Bibliographical note

ASJC Scopus Subject Areas

PubMed: MeSH publication types

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