Resumen
Single-cell RNA sequencing (scRNA-seq) is an innovative technology that can be used to characterize transcriptome heterogeneity at the single-cell resolution. The Fluidigm C1 is an automated system for preparing complementary deoxyribonucleic acid (cDNA) samples that have been synthesized and preamplified from single cells for next-generation sequencing (NGS) analysis. Herein, we detail a workflow and protocol for scRNA-seq analysis of 3'-end enriched cDNA libraries and introduce strategies for validation of differential gene expression.
Idioma original | English |
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Páginas (desde-hasta) | 261-278 |
Número de páginas | 18 |
Publicación | Methods in Molecular Biology |
Volumen | 2508 |
DOI | |
Estado | Published - 2022 |
Nota bibliográfica
Publisher Copyright:© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
ASJC Scopus Subject Areas
- Molecular Biology
- Genetics
PubMed: MeSH publication types
- Journal Article
- Research Support, Non-U.S. Gov't