Single-Cell RNA Sequencing Analysis Using Fluidigm C1 Platform for Characterization of Heterogeneous Transcriptomes

Jinhong Kim; Paola A. Marignani

doi:10.1007/978-1-0716-2376-3_19

Single-Cell RNA Sequencing Analysis Using Fluidigm C1 Platform for Characterization of Heterogeneous Transcriptomes

Jinhong Kim, Paola A. Marignani

Medicine

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

2 Citas (Scopus)

Resumen

Single-cell RNA sequencing (scRNA-seq) is an innovative technology that can be used to characterize transcriptome heterogeneity at the single-cell resolution. The Fluidigm C1 is an automated system for preparing complementary deoxyribonucleic acid (cDNA) samples that have been synthesized and preamplified from single cells for next-generation sequencing (NGS) analysis. Herein, we detail a workflow and protocol for scRNA-seq analysis of 3'-end enriched cDNA libraries and introduce strategies for validation of differential gene expression.

Idioma original	English
Páginas (desde-hasta)	261-278
Número de páginas	18
Publicación	Methods in Molecular Biology
Volumen	2508
DOI	https://doi.org/10.1007/978-1-0716-2376-3_19
Estado	Published - 2022

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Publisher Copyright:
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

ASJC Scopus Subject Areas

Molecular Biology
Genetics

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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Single-Cell RNA Sequencing Analysis Using Fluidigm C1 Platform for Characterization of Heterogeneous Transcriptomes

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