Single-Cell RNA Sequencing Analysis Using Fluidigm C1 Platform for Characterization of Heterogeneous Transcriptomes

Jinhong Kim; Paola A. Marignani

doi:10.1007/978-1-0716-2376-3_19

Single-Cell RNA Sequencing Analysis Using Fluidigm C1 Platform for Characterization of Heterogeneous Transcriptomes

Jinhong Kim, Paola A. Marignani

Medicine

Résultat de recherche: Article › examen par les pairs

2 Citations (Scopus)

Résumé

Single-cell RNA sequencing (scRNA-seq) is an innovative technology that can be used to characterize transcriptome heterogeneity at the single-cell resolution. The Fluidigm C1 is an automated system for preparing complementary deoxyribonucleic acid (cDNA) samples that have been synthesized and preamplified from single cells for next-generation sequencing (NGS) analysis. Herein, we detail a workflow and protocol for scRNA-seq analysis of 3'-end enriched cDNA libraries and introduce strategies for validation of differential gene expression.

Langue d'origine	English
Pages (de-à)	261-278
Nombre de pages	18
Journal	Methods in Molecular Biology
Volume	2508
DOI	https://doi.org/10.1007/978-1-0716-2376-3_19
Statut de publication	Published - 2022

Note bibliographique

Publisher Copyright:
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

ASJC Scopus Subject Areas

Molecular Biology
Genetics

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

Accès au document

10.1007/978-1-0716-2376-3_19

Autres fichiers et liens

Citer

@article{1463d53f03dc4fa0a0fa24ccfe66dcb2,

title = "Single-Cell RNA Sequencing Analysis Using Fluidigm C1 Platform for Characterization of Heterogeneous Transcriptomes",

abstract = "Single-cell RNA sequencing (scRNA-seq) is an innovative technology that can be used to characterize transcriptome heterogeneity at the single-cell resolution. The Fluidigm C1 is an automated system for preparing complementary deoxyribonucleic acid (cDNA) samples that have been synthesized and preamplified from single cells for next-generation sequencing (NGS) analysis. Herein, we detail a workflow and protocol for scRNA-seq analysis of 3'-end enriched cDNA libraries and introduce strategies for validation of differential gene expression.",

author = "Jinhong Kim and Marignani, {Paola A.}",

note = "Publisher Copyright: {\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2022",

doi = "10.1007/978-1-0716-2376-3_19",

language = "English",

volume = "2508",

pages = "261--278",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

publisher = "Humana Press",

}

TY - JOUR

T1 - Single-Cell RNA Sequencing Analysis Using Fluidigm C1 Platform for Characterization of Heterogeneous Transcriptomes

AU - Kim, Jinhong

AU - Marignani, Paola A.

PY - 2022

Y1 - 2022

N2 - Single-cell RNA sequencing (scRNA-seq) is an innovative technology that can be used to characterize transcriptome heterogeneity at the single-cell resolution. The Fluidigm C1 is an automated system for preparing complementary deoxyribonucleic acid (cDNA) samples that have been synthesized and preamplified from single cells for next-generation sequencing (NGS) analysis. Herein, we detail a workflow and protocol for scRNA-seq analysis of 3'-end enriched cDNA libraries and introduce strategies for validation of differential gene expression.

AB - Single-cell RNA sequencing (scRNA-seq) is an innovative technology that can be used to characterize transcriptome heterogeneity at the single-cell resolution. The Fluidigm C1 is an automated system for preparing complementary deoxyribonucleic acid (cDNA) samples that have been synthesized and preamplified from single cells for next-generation sequencing (NGS) analysis. Herein, we detail a workflow and protocol for scRNA-seq analysis of 3'-end enriched cDNA libraries and introduce strategies for validation of differential gene expression.

UR - http://www.scopus.com/inward/record.url?scp=85133100848&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85133100848&partnerID=8YFLogxK

U2 - 10.1007/978-1-0716-2376-3_19

DO - 10.1007/978-1-0716-2376-3_19

M3 - Article

C2 - 35737246

AN - SCOPUS:85133100848

SN - 1064-3745

VL - 2508

SP - 261

EP - 278

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Single-Cell RNA Sequencing Analysis Using Fluidigm C1 Platform for Characterization of Heterogeneous Transcriptomes

Résumé

Note bibliographique

ASJC Scopus Subject Areas

PubMed: MeSH publication types

Accès au document

Autres fichiers et liens

Empreinte numérique

Citer